Crystal structure of the rice branching enzyme I (BEI) in complex with maltopentaose

Kimiko Chaen, Junji Noguchi, Toshiro Omori, Yoshimitsu Kakuta, Makoto Kimura

研究成果: ジャーナルへの寄稿記事

21 引用 (Scopus)

抄録

Starch branching enzyme (SBE) catalyzes the cleavage of α-1,4-linkages and the subsequent transfer of α-1,4 glucan to form an α-1,6 branch point in amylopectin. We determined the crystal structure of the rice branching enzyme I (BEI) in complex with maltopentaose at a resolution of 2.2 å. Maltopentaose bound to a hydrophobic pocket formed by the N-terminal helix, carbohydrate-binding module 48 (CBM48), and α-amylase domain. In addition, glucose moieties could be observed at molecular surfaces on the N-terminal helix (α2) and CBM48. Amino acid residues involved in the carbohydrate bindings are highly conserved in other SBEs, suggesting their generally conserved role in substrate binding for SBEs.

元の言語英語
ページ(範囲)508-511
ページ数4
ジャーナルBiochemical and Biophysical Research Communications
424
発行部数3
DOI
出版物ステータス出版済み - 8 3 2012

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Crystal structure
Carbohydrates
1,4-alpha-Glucan Branching Enzyme
Amylopectin
Amylases
Amino Acids
Glucose
Substrates
N-acetylglucosaminyltransferase IGnT
maltopentaose
Oryza
1,4-glucan

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology

これを引用

Crystal structure of the rice branching enzyme I (BEI) in complex with maltopentaose. / Chaen, Kimiko; Noguchi, Junji; Omori, Toshiro; Kakuta, Yoshimitsu; Kimura, Makoto.

:: Biochemical and Biophysical Research Communications, 巻 424, 番号 3, 03.08.2012, p. 508-511.

研究成果: ジャーナルへの寄稿記事

Chaen, Kimiko ; Noguchi, Junji ; Omori, Toshiro ; Kakuta, Yoshimitsu ; Kimura, Makoto. / Crystal structure of the rice branching enzyme I (BEI) in complex with maltopentaose. :: Biochemical and Biophysical Research Communications. 2012 ; 巻 424, 番号 3. pp. 508-511.
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