Crystal Structures and Coordination Behavior of Aqua- and Cyano-Co(III) Tetradehydrocorrins in the Heme Pocket of Myoglobin

Yoshitsugu Morita, Koji Oohora, Eiichi Mizohata, Akiyoshi Sawada, Takashi Kamachi, Kazunari Yoshizawa, Tsuyoshi Inoue, Takashi Hayashi

研究成果: ジャーナルへの寄稿学術誌査読

10 被引用数 (Scopus)


Myoglobins reconstituted with aqua- and cyano-Co(III) tetradehydrocorrins, rMb(CoIII(OH2)(TDHC)) and rMb(CoIII(CN)(TDHC)), respectively, were prepared and investigated as models of a cobalamin-dependent enzyme. The former protein was obtained by oxidation of rMb(CoII(TDHC)) with K3[Fe(CN)6]. The cyanide-coordinated Co(III) species in the latter protein was prepared by ligand exchange of rMb(CoIII(OH2)(TDHC)) with exogenous cyanide upon addition of KCN. The X-ray crystallographic study reveals the hexacoordinated structures of rMb(CoIII(OH)(TDHC)) and rMb(CoIII(CN)(TDHC)) at 1.20 and 1.40 Å resolution, respectively. The 13C NMR chemical shifts of the cyanide in rMb(CoIII(CN)(TDHC)) were determined to be 108.6 and 110.6 ppm. IR measurements show that the cyanide of rMb(CoIII(CN)(TDHC)) has a stretching frequency peak at 2151 cm-1 which is higher than that of cyanocobalamin. The 13C NMR and IR measurements indicate weaker coordination of the cyanide to CoIII(TDHC) relative to cobalamin, a vitamin B12 derivative. Thus, the extent of π-back-donation from the cobalt ion to the cyanide ion is lower in rMb(CoIII(CN)(TDHC)). Furthermore, the pK1/2 values of rMb(CoIII(OH2)(TDHC)) and rMb(CoIII(CN)(TDHC)) were determined by a pH titration experiment to be 3.2 and 5.5, respectively, indicating that the cyanide ligation weakens the Co-N(His93) bond. Theoretical calculations also demonstrate that the axial ligand exchange from water to cyanide elongates the Co-N(axial) bond with a decrease in the bond dissociation energy. Taken together, the cyano-Co(III) tetradehydrocorrin in myoglobin is appropriate for investigation as a structural analogue of methylcobalamin, a key intermediate in methionine synthase reaction.

ジャーナルInorganic chemistry
出版ステータス出版済み - 2月 1 2016

!!!All Science Journal Classification (ASJC) codes

  • 物理化学および理論化学
  • 無機化学


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