Crystal structures of the HypCD complex and the HypCDE ternary complex: Transient intermediate complexes during [NiFe] hydrogenase maturation

Satoshi Watanabe, Rie Matsumi, Haruyuki Atomi, Tadayuki Imanaka, Kunio Miki

研究成果: Contribution to journalArticle査読

30 被引用数 (Scopus)

抄録

[NiFe] hydrogenase maturation represents one of the most dynamic and sophisticated processes in metallocenter assembly. The Fe(CN)2CO moiety of [NiFe] hydrogenases is assembled via unknown transient interactions among specific maturation proteins HypC (metallochaperone), HypD (redox protein), and HypE (cyanide synthesis/donor). Here, we report the structures of the HypC-HypD and HypC-HypD-HypE complexes, providing a view of the transient interactions that take place during the maturation process. HypC binds to the conserved region of HypD through extensive hydrophobic interactions. The ternary complex formation between HypE and the HypCD complex involves both HypC and HypD, rendering the HypE conformation favorable for cyanide transfer. In the complex, the conserved cysteines of HypC and HypD form an Fe binding site. The conserved C-terminal cysteine of HypE can access the thiol redox cascade of HypD. These results provide structural insights into the Fe atom cyanation in the HypCDE complex.

本文言語英語
ページ(範囲)2124-2137
ページ数14
ジャーナルStructure
20
12
DOI
出版ステータス出版済み - 12 5 2012
外部発表はい

All Science Journal Classification (ASJC) codes

  • 構造生物学
  • 分子生物学

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