Crystallization and preliminary crystallographic study of DNA polymerase from Pyrococcus furiosus

Hirokazu Nishida, Maiko Tanabe, Yoshizumi Ishino, Takuji Oyama, Kosuke Morikawa

研究成果: Contribution to journalArticle査読

3 被引用数 (Scopus)

抄録

A new member of archaeal DNA polymerase from Pyrococcus furiosus was crystallized. Diffraction data to 3.1 Å of the selenomethionine- derivatized crystal were collected, and preliminary crystallographic study has been completed. The crystal belongs to the space group C2 with unit cell parameters of a = 93.2 Å, b = 124.9 Å, c = 87.7 Å, α = 90° , β = 109.7° , and γ = 90°. Assuming the presence of one molecule in the asymmetric unit, the solvent content of the crystal is estimated to be 54%, corresponding to a Matthews coefficient VM of 2.7Å3 Da-1.

本文言語英語
ページ(範囲)403-405
ページ数3
ジャーナルProtein and Peptide Letters
14
4
DOI
出版ステータス出版済み - 4 2007

All Science Journal Classification (ASJC) codes

  • 構造生物学
  • 生化学

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