Crystallization and preliminary X-ray analysis of human MTH1 complexed with two oxidized nucleotides, 8-oxo-dGMP and 2-oxo-dATP

Teruya Nakamura, Yuki Kitaguchi, Masayuki Miyazawa, Hiroyuki Kamiya, Sachiko Toma, Shinji Ikemizu, Masahiro Shirakawa, Yusaku Nakabeppu, Yuriko Yamagata

研究成果: ジャーナルへの寄稿記事

5 引用 (Scopus)

抄録

Human MutT homologue 1 (hMTH1) hydrolyzes a variety of oxidized purine nucleoside triphosphates, including 8-oxo-dGTP, 2-oxo-dATP, 2-oxo-ATP and 8-oxo-dATP, to their corresponding nucleoside monophosphates, while Esherichia coli MutT possesses prominent substrate specificity for 8-oxoguanine nucleotides. Three types of crystals were obtained corresponding to the following complexes: selenomethionine-labelled hMTH1 with 8-oxo-dGMP (SeMet hMTH1-8-oxo-dGMP), hMTH1 with 8-oxo-dGMP (hMTH1-8-oxo-dGMP) and hMTH1 with 2-oxo-dATP (hMTH1-2-oxo-dATP). Crystals of the SeMet hMTH1-8-oxo-dGMP complex belong to space group P41212, with unit-cell parameters a = b = 45.8, c = 153.6 Å, and diffracted to 2.90 Å. Crystals of hMTH1-8-oxo-dGMP and hMTH1-2-oxo-dATP belong to space groups P21 and P212121, with unit-cell parameters a = 34.0, b = 59.0, c = 65.9 Å, β = 90.7° and a = 59.2, b = 67.3, c = 80.0 Å, respectively. Their diffraction data were collected at resolutions of 1.95 and 2.22 Å, respectively.

元の言語英語
ページ(範囲)1283-1285
ページ数3
ジャーナルActa Crystallographica Section F: Structural Biology and Crystallization Communications
62
発行部数12
DOI
出版物ステータス出版済み - 12 1 2006

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X ray analysis
nucleotides
Crystallization
nucleosides
Nucleotides
X-Rays
crystallization
crystals
purines
x rays
adenosine triphosphate
cells
Crystals
Selenomethionine
Purine Nucleosides
Nucleosides
diffraction
8-oxo-7,8-dihydro-2'-deoxyguanosine-5'-monophosphate
Diffraction
Substrate Specificity

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Genetics
  • Condensed Matter Physics

これを引用

Crystallization and preliminary X-ray analysis of human MTH1 complexed with two oxidized nucleotides, 8-oxo-dGMP and 2-oxo-dATP. / Nakamura, Teruya; Kitaguchi, Yuki; Miyazawa, Masayuki; Kamiya, Hiroyuki; Toma, Sachiko; Ikemizu, Shinji; Shirakawa, Masahiro; Nakabeppu, Yusaku; Yamagata, Yuriko.

:: Acta Crystallographica Section F: Structural Biology and Crystallization Communications, 巻 62, 番号 12, 01.12.2006, p. 1283-1285.

研究成果: ジャーナルへの寄稿記事

Nakamura, Teruya ; Kitaguchi, Yuki ; Miyazawa, Masayuki ; Kamiya, Hiroyuki ; Toma, Sachiko ; Ikemizu, Shinji ; Shirakawa, Masahiro ; Nakabeppu, Yusaku ; Yamagata, Yuriko. / Crystallization and preliminary X-ray analysis of human MTH1 complexed with two oxidized nucleotides, 8-oxo-dGMP and 2-oxo-dATP. :: Acta Crystallographica Section F: Structural Biology and Crystallization Communications. 2006 ; 巻 62, 番号 12. pp. 1283-1285.
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abstract = "Human MutT homologue 1 (hMTH1) hydrolyzes a variety of oxidized purine nucleoside triphosphates, including 8-oxo-dGTP, 2-oxo-dATP, 2-oxo-ATP and 8-oxo-dATP, to their corresponding nucleoside monophosphates, while Esherichia coli MutT possesses prominent substrate specificity for 8-oxoguanine nucleotides. Three types of crystals were obtained corresponding to the following complexes: selenomethionine-labelled hMTH1 with 8-oxo-dGMP (SeMet hMTH1-8-oxo-dGMP), hMTH1 with 8-oxo-dGMP (hMTH1-8-oxo-dGMP) and hMTH1 with 2-oxo-dATP (hMTH1-2-oxo-dATP). Crystals of the SeMet hMTH1-8-oxo-dGMP complex belong to space group P41212, with unit-cell parameters a = b = 45.8, c = 153.6 {\AA}, and diffracted to 2.90 {\AA}. Crystals of hMTH1-8-oxo-dGMP and hMTH1-2-oxo-dATP belong to space groups P21 and P212121, with unit-cell parameters a = 34.0, b = 59.0, c = 65.9 {\AA}, β = 90.7° and a = 59.2, b = 67.3, c = 80.0 {\AA}, respectively. Their diffraction data were collected at resolutions of 1.95 and 2.22 {\AA}, respectively.",
author = "Teruya Nakamura and Yuki Kitaguchi and Masayuki Miyazawa and Hiroyuki Kamiya and Sachiko Toma and Shinji Ikemizu and Masahiro Shirakawa and Yusaku Nakabeppu and Yuriko Yamagata",
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AU - Nakamura, Teruya

AU - Kitaguchi, Yuki

AU - Miyazawa, Masayuki

AU - Kamiya, Hiroyuki

AU - Toma, Sachiko

AU - Ikemizu, Shinji

AU - Shirakawa, Masahiro

AU - Nakabeppu, Yusaku

AU - Yamagata, Yuriko

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AB - Human MutT homologue 1 (hMTH1) hydrolyzes a variety of oxidized purine nucleoside triphosphates, including 8-oxo-dGTP, 2-oxo-dATP, 2-oxo-ATP and 8-oxo-dATP, to their corresponding nucleoside monophosphates, while Esherichia coli MutT possesses prominent substrate specificity for 8-oxoguanine nucleotides. Three types of crystals were obtained corresponding to the following complexes: selenomethionine-labelled hMTH1 with 8-oxo-dGMP (SeMet hMTH1-8-oxo-dGMP), hMTH1 with 8-oxo-dGMP (hMTH1-8-oxo-dGMP) and hMTH1 with 2-oxo-dATP (hMTH1-2-oxo-dATP). Crystals of the SeMet hMTH1-8-oxo-dGMP complex belong to space group P41212, with unit-cell parameters a = b = 45.8, c = 153.6 Å, and diffracted to 2.90 Å. Crystals of hMTH1-8-oxo-dGMP and hMTH1-2-oxo-dATP belong to space groups P21 and P212121, with unit-cell parameters a = 34.0, b = 59.0, c = 65.9 Å, β = 90.7° and a = 59.2, b = 67.3, c = 80.0 Å, respectively. Their diffraction data were collected at resolutions of 1.95 and 2.22 Å, respectively.

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