Crystallization and X-ray diffraction analysis of chondroitin lyase from baculovirus: Envelope protein ODV-E66

Yoshirou Kawaguchi, Nobuo Sugiura, Momo Onishi, Koji Kimata, Makoto Kimura, Yoshimitsu Kakuta

研究成果: ジャーナルへの寄稿記事

3 引用 (Scopus)

抄録

Baculovirus envelope protein ODV-E66 (67-704), in which the N-terminal 66 amino acids are truncated, is a chondroitin lyase. It digests chondroitin and chondroitin 6-sulfate efficiently, but does not digest chondroitin 4-sulfate. This unique characteristic is useful for the preparation of specific chondroitin oligosaccharides and for investigation of the mechanism of baculovirus infection. ODV-E66 (67-704) was crystallized; the crystal diffracted to 1.8 Å resolution and belonged to space group P62 or P64, with unit-cell parameters a = b = 113.5, c = 101.5 Å. One molecule is assumed to be present per asymmetric unit, which gives a Matthews coefficient of 2.54 Å 3 Da -1.

元の言語英語
ページ(範囲)190-192
ページ数3
ジャーナルActa Crystallographica Section F: Structural Biology and Crystallization Communications
68
発行部数2
DOI
出版物ステータス出版済み - 2 1 2012

Fingerprint

Chondroitin Lyases
Chondroitin
Chondroitin Sulfates
Baculoviridae
Crystallization
X-Ray Diffraction
X ray diffraction analysis
sulfates
envelopes
crystallization
proteins
infectious diseases
Oligosaccharides
diffraction
amino acids
Proteins
x rays
Amino Acids
preparation
Crystals

All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Biophysics
  • Biochemistry
  • Genetics
  • Condensed Matter Physics

これを引用

Crystallization and X-ray diffraction analysis of chondroitin lyase from baculovirus : Envelope protein ODV-E66. / Kawaguchi, Yoshirou; Sugiura, Nobuo; Onishi, Momo; Kimata, Koji; Kimura, Makoto; Kakuta, Yoshimitsu.

:: Acta Crystallographica Section F: Structural Biology and Crystallization Communications, 巻 68, 番号 2, 01.02.2012, p. 190-192.

研究成果: ジャーナルへの寄稿記事

@article{048af5802ee64d81ba2a8949aec92c1e,
title = "Crystallization and X-ray diffraction analysis of chondroitin lyase from baculovirus: Envelope protein ODV-E66",
abstract = "Baculovirus envelope protein ODV-E66 (67-704), in which the N-terminal 66 amino acids are truncated, is a chondroitin lyase. It digests chondroitin and chondroitin 6-sulfate efficiently, but does not digest chondroitin 4-sulfate. This unique characteristic is useful for the preparation of specific chondroitin oligosaccharides and for investigation of the mechanism of baculovirus infection. ODV-E66 (67-704) was crystallized; the crystal diffracted to 1.8 {\AA} resolution and belonged to space group P62 or P64, with unit-cell parameters a = b = 113.5, c = 101.5 {\AA}. One molecule is assumed to be present per asymmetric unit, which gives a Matthews coefficient of 2.54 {\AA} 3 Da -1.",
author = "Yoshirou Kawaguchi and Nobuo Sugiura and Momo Onishi and Koji Kimata and Makoto Kimura and Yoshimitsu Kakuta",
year = "2012",
month = "2",
day = "1",
doi = "10.1107/S1744309111053164",
language = "English",
volume = "68",
pages = "190--192",
journal = "Acta Crystallographica Section F:Structural Biology Communications",
issn = "1744-3091",
publisher = "John Wiley and Sons Ltd",
number = "2",

}

TY - JOUR

T1 - Crystallization and X-ray diffraction analysis of chondroitin lyase from baculovirus

T2 - Envelope protein ODV-E66

AU - Kawaguchi, Yoshirou

AU - Sugiura, Nobuo

AU - Onishi, Momo

AU - Kimata, Koji

AU - Kimura, Makoto

AU - Kakuta, Yoshimitsu

PY - 2012/2/1

Y1 - 2012/2/1

N2 - Baculovirus envelope protein ODV-E66 (67-704), in which the N-terminal 66 amino acids are truncated, is a chondroitin lyase. It digests chondroitin and chondroitin 6-sulfate efficiently, but does not digest chondroitin 4-sulfate. This unique characteristic is useful for the preparation of specific chondroitin oligosaccharides and for investigation of the mechanism of baculovirus infection. ODV-E66 (67-704) was crystallized; the crystal diffracted to 1.8 Å resolution and belonged to space group P62 or P64, with unit-cell parameters a = b = 113.5, c = 101.5 Å. One molecule is assumed to be present per asymmetric unit, which gives a Matthews coefficient of 2.54 Å 3 Da -1.

AB - Baculovirus envelope protein ODV-E66 (67-704), in which the N-terminal 66 amino acids are truncated, is a chondroitin lyase. It digests chondroitin and chondroitin 6-sulfate efficiently, but does not digest chondroitin 4-sulfate. This unique characteristic is useful for the preparation of specific chondroitin oligosaccharides and for investigation of the mechanism of baculovirus infection. ODV-E66 (67-704) was crystallized; the crystal diffracted to 1.8 Å resolution and belonged to space group P62 or P64, with unit-cell parameters a = b = 113.5, c = 101.5 Å. One molecule is assumed to be present per asymmetric unit, which gives a Matthews coefficient of 2.54 Å 3 Da -1.

UR - http://www.scopus.com/inward/record.url?scp=84856897469&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84856897469&partnerID=8YFLogxK

U2 - 10.1107/S1744309111053164

DO - 10.1107/S1744309111053164

M3 - Article

C2 - 22297996

AN - SCOPUS:84856897469

VL - 68

SP - 190

EP - 192

JO - Acta Crystallographica Section F:Structural Biology Communications

JF - Acta Crystallographica Section F:Structural Biology Communications

SN - 1744-3091

IS - 2

ER -