CUE domain-containing protein vps901 is required for vacuolar protein transport in Schizosaccharomyces pombe

Mai Nakase, Yuta Tsukamoto, Akira Hosomi, Tomo Matsuda, Masaaki Miyamoto, Kaoru Takegawa

研究成果: ジャーナルへの寄稿記事

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The functions of two Schizosaccharomyces pombe Vps9- like genes, SPBC4F6.10/vps901+ and SPBC29A10.11c/ vps902+, were characterized. Genomic sequence analysis predicted that Vps901p contains a VPS9 domain, whereas cDNA analyses revealed that Vps901p contains a CUE domain (coupling of ubiquitin to ER degradation) in its C-terminal region. Deletion of vps901+ resulted in mis-sorting and secretion of S. pombe vacuolar carboxypeptidase Cpy1p, whereas deletion of vps902+ had no effect, suggesting that only Vps901p functions in vacuolar protein transport in S. pombe. Deletion of vps901+ further produced pleiotropic phenotypes, including vacuolar homotypic fusion and endocytosis defects. Heterologous expression of the budding yeast VPS9 gene corrected the CPY mis-sorting defect in vps901Δ cells. These findings suggest that the VPS9 domain of Vps901p is required for vacuolar protein trafficking in S. pombe.

元の言語英語
ページ(範囲)652-659
ページ数8
ジャーナルBioscience, Biotechnology and Biochemistry
76
発行部数4
DOI
出版物ステータス出版済み - 4 30 2012

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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