TY - JOUR
T1 - Cultivation of spore-forming gut microbes using a combination of bile acids and amino acids
AU - Onizuka, Sakura
AU - Tanaka, Masaru
AU - Mishima, Riko
AU - Nakayama, Jiro
N1 - Funding Information:
Funding: This study was supported by JSPS KAKENHI grant numbers JP 17H04620 and 20KK0130 (to J.N.), funding from the Institute for Fermentation, Osaka (IFO), Japan (to J.N.) and funding from Nagase Science Technology Foundation, Japan (to J.N.).
Publisher Copyright:
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2021/8
Y1 - 2021/8
N2 - Spores of certain species belonging to Firmicutes are efficiently germinated by nutrient germinators, such as amino acids, in addition to bile acid. We attempted to culture difficult-to-culture or yet-to-be cultured spore-forming intestinal bacteria, using a combination of bile acids and amino acids. The combination increased the number of colonies that formed on agar medium plated with ethanol-treated feces. The operational taxonomic units of these colonized bacteria were classified into two types. One type was colonized only by the bile acid (BA) mixture and the other type was colonized using amino acids, in addition to the BA mixture. The latter contained 13 species, in addition to 14 species of the former type, which mostly corresponds to anaerobic difficult-to-culture Clostridiales species, including several new species candidates. The use of a combination of BAs and amino acids effectively increased the culturability of spore-forming intestinal bacteria.
AB - Spores of certain species belonging to Firmicutes are efficiently germinated by nutrient germinators, such as amino acids, in addition to bile acid. We attempted to culture difficult-to-culture or yet-to-be cultured spore-forming intestinal bacteria, using a combination of bile acids and amino acids. The combination increased the number of colonies that formed on agar medium plated with ethanol-treated feces. The operational taxonomic units of these colonized bacteria were classified into two types. One type was colonized only by the bile acid (BA) mixture and the other type was colonized using amino acids, in addition to the BA mixture. The latter contained 13 species, in addition to 14 species of the former type, which mostly corresponds to anaerobic difficult-to-culture Clostridiales species, including several new species candidates. The use of a combination of BAs and amino acids effectively increased the culturability of spore-forming intestinal bacteria.
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U2 - 10.3390/microorganisms9081651
DO - 10.3390/microorganisms9081651
M3 - Article
AN - SCOPUS:85111455562
VL - 9
JO - Microorganisms
JF - Microorganisms
SN - 2076-2607
IS - 8
M1 - 1651
ER -