Cyanobacterial production of 1,3-propanediol directly from carbon dioxide using a synthetic metabolic pathway

Yasutaka Hirokawa, Yuki Maki, Tsuneyuki Tatsuke, Taizo Hanai

研究成果: ジャーナルへの寄稿記事

31 引用 (Scopus)

抄録

Production of chemicals directly from carbon dioxide using light energy is an attractive option for a sustainable future. The 1,3-propanediol (1,3-PDO) production directly from carbon dioxide was achieved by engineered Synechococcus elongatus PCC 7942 with a synthetic metabolic pathway. Glycerol dehydratase catalyzing the conversion of glycerol to 3-hydroxypropionaldehyde in a coenzyme B12-dependent manner worked in S. elongatus PCC 7942 without addition of vitamin B12, suggesting that the intrinsic pseudovitamin B12 served as a substitute of coenzyme B12. The highest titers of 1,3-PDO (3.79±0.23mM; 288±17.7mg/L) and glycerol (12.62±1.55mM; 1.16±0.14g/L), precursor of 1,3-PDO, were reached after 14 days of culture under optimized conditions in this study.

元の言語英語
ページ(範囲)97-103
ページ数7
ジャーナルMetabolic Engineering
34
DOI
出版物ステータス出版済み - 3 1 2016

Fingerprint

Metabolic Networks and Pathways
Glycerol
Carbon Dioxide
Coenzymes
Carbon dioxide
glycerol dehydratase
Synechococcus
Vitamins
Vitamin B 12
Light
1,3-propanediol
cobamamide

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

これを引用

Cyanobacterial production of 1,3-propanediol directly from carbon dioxide using a synthetic metabolic pathway. / Hirokawa, Yasutaka; Maki, Yuki; Tatsuke, Tsuneyuki; Hanai, Taizo.

:: Metabolic Engineering, 巻 34, 01.03.2016, p. 97-103.

研究成果: ジャーナルへの寄稿記事

@article{e20d346ca724413190b77992254b7bda,
title = "Cyanobacterial production of 1,3-propanediol directly from carbon dioxide using a synthetic metabolic pathway",
abstract = "Production of chemicals directly from carbon dioxide using light energy is an attractive option for a sustainable future. The 1,3-propanediol (1,3-PDO) production directly from carbon dioxide was achieved by engineered Synechococcus elongatus PCC 7942 with a synthetic metabolic pathway. Glycerol dehydratase catalyzing the conversion of glycerol to 3-hydroxypropionaldehyde in a coenzyme B12-dependent manner worked in S. elongatus PCC 7942 without addition of vitamin B12, suggesting that the intrinsic pseudovitamin B12 served as a substitute of coenzyme B12. The highest titers of 1,3-PDO (3.79±0.23mM; 288±17.7mg/L) and glycerol (12.62±1.55mM; 1.16±0.14g/L), precursor of 1,3-PDO, were reached after 14 days of culture under optimized conditions in this study.",
author = "Yasutaka Hirokawa and Yuki Maki and Tsuneyuki Tatsuke and Taizo Hanai",
year = "2016",
month = "3",
day = "1",
doi = "10.1016/j.ymben.2015.12.008",
language = "English",
volume = "34",
pages = "97--103",
journal = "Metabolic Engineering",
issn = "1096-7176",
publisher = "Academic Press Inc.",

}

TY - JOUR

T1 - Cyanobacterial production of 1,3-propanediol directly from carbon dioxide using a synthetic metabolic pathway

AU - Hirokawa, Yasutaka

AU - Maki, Yuki

AU - Tatsuke, Tsuneyuki

AU - Hanai, Taizo

PY - 2016/3/1

Y1 - 2016/3/1

N2 - Production of chemicals directly from carbon dioxide using light energy is an attractive option for a sustainable future. The 1,3-propanediol (1,3-PDO) production directly from carbon dioxide was achieved by engineered Synechococcus elongatus PCC 7942 with a synthetic metabolic pathway. Glycerol dehydratase catalyzing the conversion of glycerol to 3-hydroxypropionaldehyde in a coenzyme B12-dependent manner worked in S. elongatus PCC 7942 without addition of vitamin B12, suggesting that the intrinsic pseudovitamin B12 served as a substitute of coenzyme B12. The highest titers of 1,3-PDO (3.79±0.23mM; 288±17.7mg/L) and glycerol (12.62±1.55mM; 1.16±0.14g/L), precursor of 1,3-PDO, were reached after 14 days of culture under optimized conditions in this study.

AB - Production of chemicals directly from carbon dioxide using light energy is an attractive option for a sustainable future. The 1,3-propanediol (1,3-PDO) production directly from carbon dioxide was achieved by engineered Synechococcus elongatus PCC 7942 with a synthetic metabolic pathway. Glycerol dehydratase catalyzing the conversion of glycerol to 3-hydroxypropionaldehyde in a coenzyme B12-dependent manner worked in S. elongatus PCC 7942 without addition of vitamin B12, suggesting that the intrinsic pseudovitamin B12 served as a substitute of coenzyme B12. The highest titers of 1,3-PDO (3.79±0.23mM; 288±17.7mg/L) and glycerol (12.62±1.55mM; 1.16±0.14g/L), precursor of 1,3-PDO, were reached after 14 days of culture under optimized conditions in this study.

UR - http://www.scopus.com/inward/record.url?scp=84954288565&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84954288565&partnerID=8YFLogxK

U2 - 10.1016/j.ymben.2015.12.008

DO - 10.1016/j.ymben.2015.12.008

M3 - Article

VL - 34

SP - 97

EP - 103

JO - Metabolic Engineering

JF - Metabolic Engineering

SN - 1096-7176

ER -