Cyclin A Is Redundant in Fibroblasts but Essential in Hematopoietic and Embryonic Stem Cells

Ilona Kalaszczynska, Yan Geng, Tadafumi Iino, Shin ichi Mizuno, Yoon Choi, Ilona Kondratiuk, Daniel P. Silver, Debra J. Wolgemuth, Koichi Akashi, Piotr Sicinski

研究成果: Contribution to journalArticle査読

156 被引用数 (Scopus)

抄録

Cyclins are regulatory subunits of cyclin-dependent kinases. Cyclin A, the first cyclin ever cloned, is thought to be an essential component of the cell-cycle engine. Mammalian cells encode two A-type cyclins, testis-specific cyclin A1 and ubiquitously expressed cyclin A2. Here, we tested the requirement for cyclin A function using conditional knockout mice lacking both A-type cyclins. We found that acute ablation of cyclin A in fibroblasts did not affect cell proliferation, but led to prolonged expression of another cyclin, cyclin E, across the cell cycle. However, combined ablation of all A- and E-type cyclins extinguished cell division. In contrast, cyclin A function was essential for cell-cycle progression of hematopoietic and embryonic stem cells. Expression of cyclin A is particularly high in these compartments, which might render stem cells dependent on cyclin A, whereas in fibroblasts cyclins A and E play redundant roles in cell proliferation.

本文言語英語
ページ(範囲)352-365
ページ数14
ジャーナルCell
138
2
DOI
出版ステータス出版済み - 7 23 2009
外部発表はい

All Science Journal Classification (ASJC) codes

  • 生化学、遺伝学、分子生物学(全般)

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