Cyclin D2 translocates p27 out of the nucleus and promotes its degradation at the G0-G1 transition

Etsuo Susaki, Keiko Nakayama, Keiichi I. Nakayama

研究成果: Contribution to journalArticle査読

53 被引用数 (Scopus)

抄録

The nuclear export and cytoplasmic degradation of the cyclin-dependent kinase inhibitor p27 are required for effective progression of the cell cycle through the G0-G1 transition. The mechanism responsible for this translocation of p27 has remained unclear, however. We now show that cyclin D2 directly links growth signaling with the nuclear export of p27 at the G0-G1 transition in some cell types. The up-regulation of cyclin D2 in response to mitogenic stimulation was found to occur earlier than that of other D-type cyclins and in parallel with down-regulation of p27 at the G0-G1 transition. RNA interference-mediated depletion of cyclin D2 inhibited the nuclear export of p27 and delayed its degradation at the G0-G1 transition. In contrast, overexpression of cyclin D2 in G0 phase shifted the localization of p27 from the nucleus to the cytoplasm and reduced the stability of p27. Overexpression of the cyclin D2(T280A) mutant, whose export from the nucleus is impaired, prevented the translocation and degradation of p27. These results indicate that cyclin D2 translocates p27 from the nucleus into the cytoplasm for its KPC-dependent degradation at the G0-G1 transition.

本文言語英語
ページ(範囲)4626-4640
ページ数15
ジャーナルMolecular and cellular biology
27
13
DOI
出版ステータス出版済み - 7 2007

All Science Journal Classification (ASJC) codes

  • 分子生物学
  • 細胞生物学

フィンガープリント

「Cyclin D2 translocates p27 out of the nucleus and promotes its degradation at the G<sub>0</sub>-G<sub>1</sub> transition」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル