In this study, we evaluated the cytocompatibility of high-pressure torsion (HPT)-processed Ti-6Al-7Nb alloys. Grain size of titanium (Ti) alloys decreased from 5 μm (without HPT processing, HPT-0) to ∼100 nm (at 2 GPa, HPT-2) or ∼70 nm (at 6 GPa, HPT-6) through HPT processing. Cell adhesion, proliferation, and differentiation were evaluated with mouse preosteoblast (MC3T3-E1). A locomotion trend was presented by cells cultured on HPT-2 to compare with other specimens, while, an immobilization trend was presented by cells cultured on HPT-6 to compare with other specimens. Akp2 (alkaline phosphatase 2) expression was higher in cells cultured on HPT-2 than that on HPT-0 and HPT-6, indicating enhanced osteogenic differentiation. Our results demonstrate that HPT-processed Ti-6Al-7Nb alloys offer good cytocompatibility.
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