Cytoplasmic transfer between adhered cells by cell fusion through microslit

K. I. Wada, E. Kondo, K. Hosokawa, Y. Ito, M. Maeda

研究成果: Chapter in Book/Report/Conference proceedingConference contribution

抄録

This paper describes a novel cell fusion method which induces cell fusion between adhered cells through a microslit for preventing nuclear mixing. For this purpose, a microfluidic device which had 105 cell paring structures (CPSs) making cell pairs through microslits with 2.1 ± 0.3 μm width was fabricated. After trapping NIH3T3 cells with hydrodynamic forces at the CPSs, the cells were fused through the microslit by the Sendai virus envelope method. With following timelapse observation, we discovered that the spread cells were much less susceptible to nuclear migration passing through the microslit compared with round cells. This finding will provide an effective method for cell fusion without nuclear mixing, and will lead to an efficient method for reprogramming and transdifferentiation of target cells toward regenerative medicine.

本文言語英語
ホスト出版物のタイトル17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
出版社Chemical and Biological Microsystems Society
ページ1329-1331
ページ数3
ISBN(印刷版)9781632666246
出版ステータス出版済み - 2013
外部発表はい
イベント17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 - Freiburg, ドイツ
継続期間: 10 27 201310 31 2013

出版物シリーズ

名前17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
2

その他

その他17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
国/地域ドイツ
CityFreiburg
Period10/27/1310/31/13

All Science Journal Classification (ASJC) codes

  • バイオエンジニアリング

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