Cytotoxicity induced by erythrina variegata serine proteinase inhibitors in tumor hematopoietic stem cell lines

Hideki Ohba, Masaaki Nishikawa, Makoto Kimura, Nobuyuki Yamasaki, Sawako Moriwaki, Kyogo Itoh

研究成果: Contribution to journalArticle査読

7 被引用数 (Scopus)

抄録

Based on the soluble MTT tetrazolium/formazan assay, we evaluated the cytotoxicity of Erythrina variegata proteinase inhibitors in some tumor hematopoietic stem cell lines. Among the proteinase inhibitors, EBI, which belongs to the Bowman-Birk family of inhibitors, was cytotoxic in relatively differentiated cells such as Molt4 and Jurkat derived from acute T lymphoblastic leukemia (T-ALL) cells specifically, but ETIa and ECI, which are classified into Kunitz family inhibitors, did not. It was suggested that the differences in the cytotoxicity might be due to the molecular size of the inhibitors. The succinylation of lysine residue(s) of EBI led to about 50% loss of the trypsin inhibitory activity as compared with the authentic EBI. When Molt4 cells were incubated with this derivative, no significant cytotoxicity was observed. This suggests that the proteinase inhibitory activity might be involved in the cytotoxicity in human tumor cell lines.

本文言語英語
ページ(範囲)1166-1170
ページ数5
ジャーナルBioscience, Biotechnology and Biochemistry
62
6
DOI
出版ステータス出版済み - 1 1 1998

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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