DAAM1 stabilizes epithelial junctions by restraining WAVE complex-dependent lateral membrane motility

Tamako Nishimura, Shoko Ito, Hiroko Saito, Sylvain Hiver, Kenta Shigetomi, Junichi Ikenouchi, Masatoshi Takeichi

研究成果: Contribution to journalArticle査読

15 被引用数 (Scopus)

抄録

Epithelial junctions comprise two subdomains, the apical junctional complex (AJC) and the adjacent lateral membrane contacts (LCs), that span the majority of the junction. The AJC is lined with circumferential actin cables, whereas the LCs are associated with less-organized actin filaments whose roles are elusive. We found that DAAM1, a formin family actin regulator, accumulated at the LCs, and its depletion caused dispersion of actin filaments at these sites while hardly affecting circumferential actin cables. DAAM1 loss enhanced the motility of LC-forming membranes, leading to their invasion of neighboring cell layers, as well as disruption of polarized epithelial layers. We found that components of the WAVE complex and its downstream targets were required for the elevation of LC motility caused by DAAM1 loss. These findings suggest that the LC membranes are motile by nature because of the WAVE complex, but DAAM1-mediated actin regulation normally restrains this motility, thereby stabilizing epithelial architecture, and that DAAM1 loss evokes invasive abilities of epithelial cells.

本文言語英語
ページ(範囲)559-573
ページ数15
ジャーナルJournal of Cell Biology
215
4
DOI
出版ステータス出版済み - 2016

All Science Journal Classification (ASJC) codes

  • 細胞生物学

フィンガープリント

「DAAM1 stabilizes epithelial junctions by restraining WAVE complex-dependent lateral membrane motility」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル