Daughter Cell Identity Emerges from the Interplay of Cdc42, Septins, and Exocytosis

Satoshi Okada, Marcin Leda, Julia Hanna, Natasha S. Savage, Erfei Bi, Andrew B. Goryachev

研究成果: Contribution to journalArticle査読

82 被引用数 (Scopus)

抄録

Asymmetric cell division plays a crucial role in cell differentiation, unequal replicative senescence, and stem cell maintenance. In budding yeast, the identities of mother and daughter cells begin to diverge at bud emergence when distinct plasma-membrane domains are formed and separated by a septin ring. However, the mechanisms underlying this transformation remain unknown. Here, we show that septins recruited to the site of polarization by Cdc42-GTP inhibit Cdc42 activity in a negative feedback loop, and this inhibition depends on Cdc42 GTPase-activating proteins. Combining live-cell imaging and computational modeling, we demonstrate that the septin ring is sculpted by polarized exocytosis, which creates a hole in the accumulating septin density and relieves the inhibition of Cdc42. The nascent ring generates a sharp boundary that confines the Cdc42 activity and exocytosis strictly to its enclosure and thus clearly delineates the daughter cell identity. Our findings define a fundamental mechanism underlying eukaryotic cell fate differentiation.

本文言語英語
ページ(範囲)148-161
ページ数14
ジャーナルDevelopmental Cell
26
2
DOI
出版ステータス出版済み - 7 29 2013
外部発表はい

All Science Journal Classification (ASJC) codes

  • 分子生物学
  • 生化学、遺伝学、分子生物学(全般)
  • 発生生物学
  • 細胞生物学

フィンガープリント

「Daughter Cell Identity Emerges from the Interplay of Cdc42, Septins, and Exocytosis」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル