Dax-1 as one of the target genes of Ad4BP/SF-1

Ken Kawabe, T. Shikayama, H. Tsuboi, S. Oka, K. Oba, T. Yanase, H. Nawata, Ken-Ichirou Morohashi

研究成果: ジャーナルへの寄稿記事

98 引用 (Scopus)

抄録

The DAX-1 (also known as AHC) gene encodes an unusual member of the nuclear hormone receptor superfamily. DAX-1 plays a critical role during gonadal and adrenal differentiation since mutations of the human DAX-1 gene cause X-linked adrenal hypoplasia congenita associated with hypogona-dotropic hypogonadism. In recent studies, DAX-1 was reported to function as a transcriptional suppressor of Ad4BP/SF-1, a critical transcription factor in gonadal and adrenal differentiation. With respect to implication of Ad4BP/SF-1 in the transcriptional regulation of the DAX-1 gene, inconsistent findings have been previously reported. We investigated the upstream region of the mouse Dax-1 (also known as Ahch) gene and identified a novel Ad4/SF-1 site by transient transfection and electrophoretic mobility shift assays. In addition, immunohistochemical analyses with a specific antibody to Dax-1 indicated the presence of immunoreactive cells in steroidogenic tissues, pituitary gland, and hypothalamus. Although the distributions of Dax-1 and Ad4BP/SF-1 were very similar, they were not completely identical. The expression of Dax-1 was significantly impaired in knock-out mice of the Ftz-f1 gene, which encodes Ad4BP/ SF-1. Taken together, our findings indicate that Ad4BP/SF-1 controls the transcription of the Dax-1 gene.

元の言語英語
ページ(範囲)1267-1284
ページ数18
ジャーナルMolecular Endocrinology
13
発行部数8
DOI
出版物ステータス出版済み - 1 1 1999

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Genes
Hypogonadism
Electrophoretic Mobility Shift Assay
Pituitary Gland
Cytoplasmic and Nuclear Receptors
Knockout Mice
Hypothalamus
Transfection
Transcription Factors
Mutation
Antibodies
X-linked adrenal hypoplasia congenita

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Endocrinology

これを引用

Kawabe, K., Shikayama, T., Tsuboi, H., Oka, S., Oba, K., Yanase, T., ... Morohashi, K-I. (1999). Dax-1 as one of the target genes of Ad4BP/SF-1. Molecular Endocrinology, 13(8), 1267-1284. https://doi.org/10.1210/mend.13.8.0325

Dax-1 as one of the target genes of Ad4BP/SF-1. / Kawabe, Ken; Shikayama, T.; Tsuboi, H.; Oka, S.; Oba, K.; Yanase, T.; Nawata, H.; Morohashi, Ken-Ichirou.

:: Molecular Endocrinology, 巻 13, 番号 8, 01.01.1999, p. 1267-1284.

研究成果: ジャーナルへの寄稿記事

Kawabe, K, Shikayama, T, Tsuboi, H, Oka, S, Oba, K, Yanase, T, Nawata, H & Morohashi, K-I 1999, 'Dax-1 as one of the target genes of Ad4BP/SF-1', Molecular Endocrinology, 巻. 13, 番号 8, pp. 1267-1284. https://doi.org/10.1210/mend.13.8.0325
Kawabe K, Shikayama T, Tsuboi H, Oka S, Oba K, Yanase T その他. Dax-1 as one of the target genes of Ad4BP/SF-1. Molecular Endocrinology. 1999 1 1;13(8):1267-1284. https://doi.org/10.1210/mend.13.8.0325
Kawabe, Ken ; Shikayama, T. ; Tsuboi, H. ; Oka, S. ; Oba, K. ; Yanase, T. ; Nawata, H. ; Morohashi, Ken-Ichirou. / Dax-1 as one of the target genes of Ad4BP/SF-1. :: Molecular Endocrinology. 1999 ; 巻 13, 番号 8. pp. 1267-1284.
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