De novo DNA methylation independent establishment of maternal imprint on X chromosome in mouse oocytes

Hatsune Chiba, Ryutaro Hirasawa, Masahiro Kaneda, Yuko Amakawa, En Li, Takashi Sado, Hiroyuki Sasaki

研究成果: Contribution to journalArticle査読

28 被引用数 (Scopus)

抄録

In female mouse embryos, the paternal X chromosome (Xp) is preferentially inactivated during preimplantation development and trophoblast differentiation. This imprinted X-chromosome inactivation (XCI) is partly due to an activating imprint on the maternal X chromosome (Xm), which is set during oocyte growth. However, the nature of this imprint is unknown. DNA methylation is one candidate, and therefore we examined whether disruptions of the two de novo DNA methyltransferases in growing oocytes affect imprinted XCI. We found that accumulation of histone H3 lysine-27 trimethylation, a hallmark of XCI, occurs normally on the Xp, and not on the Xm, in female blastocysts developed from the mutant oocytes. Furthermore, the allelic expression patterns of X-linked genes including Xist and Tsix were unchanged in preimplantation embryos and also in the trophoblast. These results show that a maternal disruption of the DNA methyltransferases has no effect on imprinted XCI and argue that de novo DNA methylation is dispensable for Xm imprinting. This underscores the difference between imprinted XCI and autosomal imprinting.

本文言語英語
ページ(範囲)768-774
ページ数7
ジャーナルGenesis
46
12
DOI
出版ステータス出版済み - 12 1 2008
外部発表はい

All Science Journal Classification (ASJC) codes

  • 遺伝学
  • 内分泌学
  • 細胞生物学

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