Deciphering cellular functions of protein phosphatases by comparison of gene expression profiles in Saccharomyces cerevisiae

Masataka Hirasaki, Fumika Nakamura, Kazuo Yamagishi, Minori Numamoto, Yukiko Shimada, Keigo Uehashi, Shigeru Muta, Minetaka Sugiyama, Yoshinobu Kaneko, Satoru Kuhara, Satoshi Harashima

研究成果: ジャーナルへの寄稿記事

5 引用 (Scopus)

抄録

Expression profiles of protein phosphatase (PPase) disruptants were analyzed by use of Pearson's correlation coefficient to find profiles that correlated with those of 316 Reference Gene (RG) disruptants harboring deletions in genes with known functions. Twenty-six Δppase disruptants exhibited either a positive or negative correlation with 94 RG disruptants when the p value for Pearson's correlation coefficient was > 0.2. Some of the predictions that arose from this analysis were tested experimentally and several new Δppase phenotypes were found. Notably, Δsit4 and Δsiw14 disruptants exhibited hygromycin B sensitivity, Δsit4 and Δptc1 disruptants grew slowly on glycerol medium, the Δptc1 disruptant was found to be sensitive to calcofluor white and congo red, while the Δppg1 disruptant was found to be sensitive to congo red. Because on-going analysis of expression profiles of Saccharomyces cerevisiae disruptants is rapidly generating new data, we suggest that the approach used in the present study to explore PPase function is also applicable to other genes.

元の言語英語
ページ(範囲)433-441
ページ数9
ジャーナルJournal of Bioscience and Bioengineering
109
発行部数5
DOI
出版物ステータス出版済み - 5 1 2010

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Phosphoprotein Phosphatases
Phosphatases
Transcriptome
Gene expression
Yeast
Saccharomyces cerevisiae
Congo Red
Genes
Proteins
Hygromycin B
Gene Deletion
Glycerol
Phenotype

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

これを引用

Deciphering cellular functions of protein phosphatases by comparison of gene expression profiles in Saccharomyces cerevisiae. / Hirasaki, Masataka; Nakamura, Fumika; Yamagishi, Kazuo; Numamoto, Minori; Shimada, Yukiko; Uehashi, Keigo; Muta, Shigeru; Sugiyama, Minetaka; Kaneko, Yoshinobu; Kuhara, Satoru; Harashima, Satoshi.

:: Journal of Bioscience and Bioengineering, 巻 109, 番号 5, 01.05.2010, p. 433-441.

研究成果: ジャーナルへの寄稿記事

Hirasaki, M, Nakamura, F, Yamagishi, K, Numamoto, M, Shimada, Y, Uehashi, K, Muta, S, Sugiyama, M, Kaneko, Y, Kuhara, S & Harashima, S 2010, 'Deciphering cellular functions of protein phosphatases by comparison of gene expression profiles in Saccharomyces cerevisiae', Journal of Bioscience and Bioengineering, 巻. 109, 番号 5, pp. 433-441. https://doi.org/10.1016/j.jbiosc.2009.10.023
Hirasaki, Masataka ; Nakamura, Fumika ; Yamagishi, Kazuo ; Numamoto, Minori ; Shimada, Yukiko ; Uehashi, Keigo ; Muta, Shigeru ; Sugiyama, Minetaka ; Kaneko, Yoshinobu ; Kuhara, Satoru ; Harashima, Satoshi. / Deciphering cellular functions of protein phosphatases by comparison of gene expression profiles in Saccharomyces cerevisiae. :: Journal of Bioscience and Bioengineering. 2010 ; 巻 109, 番号 5. pp. 433-441.
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