TY - JOUR
T1 - Deciphering the genes for taste receptors for fructose in drosophila
AU - Uchizono, Shun
AU - Itoh, Taichi Q.
AU - Kim, Haein
AU - Hamada, Naoki
AU - Kwon, Jae Young
AU - Tanimura, Teiichi
N1 - Funding Information:
We thank the members of the TT laboratory for helpful comments and encouragement on this work, and Kyoko Sakamoto and Makiko Hanada for technical assistance. We also thank Dr. Hubert Amrein, Dr. Seok Jun Moon, and the Bloomington Drosophila Stock Center for providing fly strains. This work was supported by JSPS KAKENHI [Grant Number JP15J04525 to S.U.].
Publisher Copyright:
© The Korean Society for Molecular and Cellular Biology. All rights reserved.
PY - 2017
Y1 - 2017
N2 - Taste sensitivity to sugars plays an essential role in the initiation of feeding behavior. In Drosophila melanogaster, recent studies have identified several gustatory receptor (Gr) genes required for sensing sweet compounds. However, it is as yet undetermined how these GRs function as taste receptors tuned to a wide range of sugars. Among sugars, fructose has been suggested to be detected by a distinct receptor from other sugars. While GR43A has been reported to sense fructose in the brain, it is not expressed in labellar gustatory receptor neurons that show taste response to fructose. In contrast, the Gr64a–Gr64f gene cluster was recently shown to be associated with fructose sensitivity. Here we sought to decipher the genes required for fructose response among Gr64a– Gr64f genes. Unexpectedly, the qPCR analyses for these genes show that labellar expression levels of Gr64d and Gr64e are higher in fructose low-sensitivity flies than in high-sensitivity flies. Moreover, gustatory nerve responses to fructose in labellar sensilla are higher in Gr64d and Gr64f mutant lines than in mutant flies of the other Gr64a–Gr64f genes. These data suggest the possibility that deletion of GR64D or GR64F may indirectly induce enhanced fructose sensitivity in the labellum. Finally, we conclude that response to fructose cannot be explained by a single one of the Gr64a–Gr64f genes.
AB - Taste sensitivity to sugars plays an essential role in the initiation of feeding behavior. In Drosophila melanogaster, recent studies have identified several gustatory receptor (Gr) genes required for sensing sweet compounds. However, it is as yet undetermined how these GRs function as taste receptors tuned to a wide range of sugars. Among sugars, fructose has been suggested to be detected by a distinct receptor from other sugars. While GR43A has been reported to sense fructose in the brain, it is not expressed in labellar gustatory receptor neurons that show taste response to fructose. In contrast, the Gr64a–Gr64f gene cluster was recently shown to be associated with fructose sensitivity. Here we sought to decipher the genes required for fructose response among Gr64a– Gr64f genes. Unexpectedly, the qPCR analyses for these genes show that labellar expression levels of Gr64d and Gr64e are higher in fructose low-sensitivity flies than in high-sensitivity flies. Moreover, gustatory nerve responses to fructose in labellar sensilla are higher in Gr64d and Gr64f mutant lines than in mutant flies of the other Gr64a–Gr64f genes. These data suggest the possibility that deletion of GR64D or GR64F may indirectly induce enhanced fructose sensitivity in the labellum. Finally, we conclude that response to fructose cannot be explained by a single one of the Gr64a–Gr64f genes.
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U2 - 10.14348/molcells.2017.0016
DO - 10.14348/molcells.2017.0016
M3 - Article
C2 - 29047261
AN - SCOPUS:85043337190
VL - 40
SP - 731
EP - 736
JO - Molecules and Cells
JF - Molecules and Cells
SN - 1016-8478
IS - 10
ER -