Decreasd Autologous Fluorescence in Flow Cytometry of Cultured Cells

Hiroharu Kawahara, Koji Yamada, Kouichi Akiyama, Hirofumi Tachibana, Sanetaka Shirahata, Hiroki Murakami

研究成果: ジャーナルへの寄稿記事

抄録

To analyse the amounts of various cellular components in cultured mammalian cells by flow cytometry, we tried to reduce the autologous fluorescence in human-human hybridoma HB 4 C 5 cells producing IgM. The autologous fluorescence of the cells decreased after repeated rinsing with phenol red-free MEM medium at 4°C before and after ethanol-fixation. The method could be useful in the analyses of various cellular components by flow cytometry.

元の言語英語
ページ(範囲)879-881
ページ数3
ジャーナルNippon Nogeikagaku Kaishi
65
発行部数5
DOI
出版物ステータス出版済み - 1 1 1991

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Flow cytometry
cultured cells
flow cytometry
Cultured Cells
Flow Cytometry
Fluorescence
Cells
fluorescence
Phenolsulfonphthalein
Hybridomas
Immunoglobulin M
Ethanol
hybridomas
cells
phenol
ethanol
methodology

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Food Science
  • Chemistry (miscellaneous)
  • Medicine (miscellaneous)

これを引用

Decreasd Autologous Fluorescence in Flow Cytometry of Cultured Cells. / Kawahara, Hiroharu; Yamada, Koji; Akiyama, Kouichi; Tachibana, Hirofumi; Shirahata, Sanetaka; Murakami, Hiroki.

:: Nippon Nogeikagaku Kaishi, 巻 65, 番号 5, 01.01.1991, p. 879-881.

研究成果: ジャーナルへの寄稿記事

Kawahara, H, Yamada, K, Akiyama, K, Tachibana, H, Shirahata, S & Murakami, H 1991, 'Decreasd Autologous Fluorescence in Flow Cytometry of Cultured Cells', Nippon Nogeikagaku Kaishi, 巻. 65, 番号 5, pp. 879-881. https://doi.org/10.1271/nogeikagaku1924.65.879
Kawahara, Hiroharu ; Yamada, Koji ; Akiyama, Kouichi ; Tachibana, Hirofumi ; Shirahata, Sanetaka ; Murakami, Hiroki. / Decreasd Autologous Fluorescence in Flow Cytometry of Cultured Cells. :: Nippon Nogeikagaku Kaishi. 1991 ; 巻 65, 番号 5. pp. 879-881.
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