Decreased miR-340 Expression in Bone Marrow is Associated with Liver Metastasis of Colorectal Cancer

Hiroshi Takeyama, Hirofumi Yamamoto, Shinya Yamashita, Xin Wu, Hidekazu Takahashi, Junichi Nishimura, Naotsugu Haraguchi, Yasuhiro Miyake, Rei Suzuki, Kohei Murata, Masayuki Ohue, Takeshi Kato, Ichiro Takemasa, Tsunekazu Mizushima, Hideshi Ishii, Koshi Mimori, Yuichiro Doki, Masaki Mori

研究成果: Contribution to journalArticle査読

56 被引用数 (Scopus)

抄録

Studies have shown the prognostic significance of disseminated tumor cells (DTC) in bone marrow of patients with colorectal cancer. However, the molecular characteristics of DTCs, including their miRNA expression profiles, remain mostly unknown. In this study, we analyzed the miRNA expression of DTCs in bone marrow. EpCAM+ bone marrow cells were collected using immunomagnetic beads after exclusion of CD14+ and CD45+ cells, then subjected to miRNA microarray analysis. Cluster analysis (7 colorectal cancer patients with liver metastasis and 12 colorectal cancer patients without liver metastasis) indicated that miR-340 and miR-542-3p expressions were significantly decreased in EpCAM+ bone marrow cells of patients with liver metastasis (P = 0.019 and 0.037, respectively). We demonstrated that pre-miR-340 administration inhibited growth of colon cancer cells and suppressed c-Met expression in vitro. In clinical samples of colorectal cancer, miR-340 was expressed at significantly lower levels in tumor tissues compared with normal mucosa. Survival analysis in 136 patients with colorectal cancer indicated that low miR-340 expression was correlated with shorter 5-year disease-free survival (P = 0.023) and poor 5-year overall survival (P = 0.046). It was of note that the colorectal cancer group with low miR-340 and high c-Met expression had the worst prognosis. We further demonstrated that systemic pre-miR-340 administration suppressed growth of pre-established HCT116 tumors in animal therapeutic models. These findings indicate that miR-340 may be useful as a novel prognostic factor and as a therapeutic tool against colorectal cancer. Our data suggest that miR-340 in bone marrow may play an important role in regulating the metastasis cascade of colorectal cancer. Mol Cancer Ther; 13(4); 976-85.

本文言語英語
ページ(範囲)976-985
ページ数10
ジャーナルMolecular cancer therapeutics
13
4
DOI
出版ステータス出版済み - 4 2014

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

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