Decreased proportion of Foxp3+CD4+ regulatory Tcells contributes to the development of hypertension in genetically hypertensive rats

Masato Katsuki, Yoshitaka Hirooka, Takuya Kishi, Kenji Sunagawa

    研究成果: Contribution to journalArticle査読

    26 被引用数 (Scopus)

    抄録

    Objective: Dysregulation of the sympathetic nervous system and the immune system has been highlighted in the pathogenesis of hypertension. Foxp3+ regulatory T (Treg) cells, which maintain immune homeostasis, have potent antihypertensive properties. We systematically explored whether Treg cells proportions are altered in stroke-prone spontaneously hypertensive rats (SHRSP) with increased sympathetic activity. Then, we focused on Treg cells in the spleen and determined whether Treg cells proportion in the spleen could be affected by splenic sympathetic input associated with the progression of hypertension. We also investigated effects of Treg cell induction on hypertension development and cardiac hypertrophy in SHRSP. Methods and Results: We evaluated Treg cells in SHRSP. Compared with normotensive Wistar-Kyoto (WKY) rats, SHRSP exhibited decreased proportions of CD4+CD25+Foxp3+ cells (Treg cells) in the spleen before the onset of hypertension. Splenic sympathetic denervation in prehypertensive SHRSP attenuated the reduction of Treg cells at 4 weeks after denervation (P<0.05) and delayed the development of hypertension in SHRSP. Interleukin (IL)- 2 and anti-IL-2 mAb complex selectively induced Treg cells in vivo (P<0.01), delayed the development of hypertension and decreased the heart weight/body weight ratio (4.64±0.06 vs. 4.25±0.05 mg/g, P<0.01) and the cross-sectional area of cardiac myocytes in SHRSP compared with vehicle-treated SHRSP (580±3.91 vs. 438±4.43mm2, P<0.0001). Conclusion: Our findings indicate that a decrease in Treg cell proportion is crucial for the development of hypertension and cardiac hypertrophy in SHRSP, which is involved in sympathetic neural input to the spleen.

    本文言語英語
    ページ(範囲)773-783
    ページ数11
    ジャーナルJournal of hypertension
    33
    4
    DOI
    出版ステータス出版済み - 4 1 2015

    All Science Journal Classification (ASJC) codes

    • 内科学
    • 生理学
    • 循環器および心血管医学

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