Decreased secretion of Paneth cell α-defensins in graft-versus-host disease

Y. Eriguchi, K. Nakamura, D. Hashimoto, S. Shimoda, N. Shimono, K. Akashi, T. Ayabe, T. Teshima

研究成果: ジャーナルへの寄稿記事

20 引用 (Scopus)

抄録

Background: Intestinal microbial ecology is actively regulated by Paneth cell-derived antimicrobial peptides, α-defensins. Graft-versus-host disease (GVHD) is a major complication of allogeneic hematopoietic stem cell transplantation (SCT). We previously demonstrated that Paneth cells are targeted by GVHD, and their expression of antimicrobial peptide α-defensins is impaired, leading to a loss of physiological diversity among the microflora and development of bloodstream infection. Herein, we evaluated whether fecal levels of α-defensins could be surrogate marker of intestinal dysbiosis. Methods: We directly measured α-defensin cryptdin-1 (Crp1) in fecal pellets of mice with GVHD by using a novel enzyme-linked immunosorbent assay. Results: Fecal levels of Crp1 were significantly decreased in mice with GVHD but unchanged in mice without GVHD after SCT. These were correlated with intestinal flora diversity. Conclusion: We demonstrate a link between reduced secretion of Paneth cell α-defensins and dysbiosis of intestinal flora in GVHD. Fecal levels of α-defensins could be surrogate markers for intestinal microbial homeostasis.

元の言語英語
ページ(範囲)702-706
ページ数5
ジャーナルTransplant Infectious Disease
17
発行部数5
DOI
出版物ステータス出版済み - 10 2015

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Paneth Cells
Defensins
Graft vs Host Disease
Dysbiosis
Biomarkers
Peptides
Hematopoietic Stem Cell Transplantation
Stem Cell Transplantation
Ecology
Homeostasis
Enzyme-Linked Immunosorbent Assay
Infection

All Science Journal Classification (ASJC) codes

  • Infectious Diseases
  • Transplantation

これを引用

Decreased secretion of Paneth cell α-defensins in graft-versus-host disease. / Eriguchi, Y.; Nakamura, K.; Hashimoto, D.; Shimoda, S.; Shimono, N.; Akashi, K.; Ayabe, T.; Teshima, T.

:: Transplant Infectious Disease, 巻 17, 番号 5, 10.2015, p. 702-706.

研究成果: ジャーナルへの寄稿記事

Eriguchi, Y. ; Nakamura, K. ; Hashimoto, D. ; Shimoda, S. ; Shimono, N. ; Akashi, K. ; Ayabe, T. ; Teshima, T. / Decreased secretion of Paneth cell α-defensins in graft-versus-host disease. :: Transplant Infectious Disease. 2015 ; 巻 17, 番号 5. pp. 702-706.
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abstract = "Background: Intestinal microbial ecology is actively regulated by Paneth cell-derived antimicrobial peptides, α-defensins. Graft-versus-host disease (GVHD) is a major complication of allogeneic hematopoietic stem cell transplantation (SCT). We previously demonstrated that Paneth cells are targeted by GVHD, and their expression of antimicrobial peptide α-defensins is impaired, leading to a loss of physiological diversity among the microflora and development of bloodstream infection. Herein, we evaluated whether fecal levels of α-defensins could be surrogate marker of intestinal dysbiosis. Methods: We directly measured α-defensin cryptdin-1 (Crp1) in fecal pellets of mice with GVHD by using a novel enzyme-linked immunosorbent assay. Results: Fecal levels of Crp1 were significantly decreased in mice with GVHD but unchanged in mice without GVHD after SCT. These were correlated with intestinal flora diversity. Conclusion: We demonstrate a link between reduced secretion of Paneth cell α-defensins and dysbiosis of intestinal flora in GVHD. Fecal levels of α-defensins could be surrogate markers for intestinal microbial homeostasis.",
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AU - Nakamura, K.

AU - Hashimoto, D.

AU - Shimoda, S.

AU - Shimono, N.

AU - Akashi, K.

AU - Ayabe, T.

AU - Teshima, T.

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