Deficiency of starch synthase IIIa and IVb alters starch granule morphology from polyhedral to spherical in rice endosperm

Yoshiko Toyosawa, Yasushi Kawagoe, Ryo Matsushima, Naoko Crofts, Masahiro Ogawa, Masako Fukuda, Toshihiro Kumamaru, Yozo Okazaki, Miyako Kusano, Kazuki Saito, Kiminori Toyooka, Mayuko Sato, Yongfeng Ai, Jay Lin Jane, Yasunori Nakamura, Naoko Fujita

研究成果: Contribution to journalArticle査読

55 被引用数 (Scopus)

抄録

Starch granule morphology differs markedly among plant species. However, the mechanisms controlling starch granule morphology have not been elucidated. Rice (Oryza sativa) endosperm produces characteristic compound-type granules containing dozens of polyhedral starch granules within an amyloplast. Some other cereal species produce simple-type granules, in which only one starch granule is present per amyloplast. A double mutant rice deficient in the starch synthase (SS) genes SSIIIa and SSIVb (ss3a ss4b) produced spherical starch granules, whereas the parental single mutants produced polyhedral starch granules similar to the wild type. The ss3a ss4b amyloplasts contained compound-type starch granules during early developmental stages, and spherical granules were separated from each other during subsequent amyloplast development and seed dehydration. Analysis of glucan chain length distribution identified overlapping roles for SSIIIa and SSIVb in amylopectin chain synthesis, with a degree of polymerization of 42 or greater. Confocal fluorescence microscopy and immunoelectron microscopy of wild-type developing rice seeds revealed that the majority of SSIVb was localized between starch granules. Therefore, we propose that SSIIIa and SSIVb have crucial roles in determining starch granule morphology and in maintaining the amyloplast envelope structure. We present a model of spherical starch granule production.

本文言語英語
ページ(範囲)1255-1270
ページ数16
ジャーナルPlant physiology
170
3
DOI
出版ステータス出版済み - 3 2016

All Science Journal Classification (ASJC) codes

  • Physiology
  • Genetics
  • Plant Science

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