Degradation of distillery lees (Shochu kasu) by cellulase-producing thraustochytrids

Yousuke Taoka, Naoki Nagano, Hiroyuki Kai, Masahiro Hayashi

研究成果: ジャーナルへの寄稿記事

2 引用 (Scopus)

抄録

Single cell oils produced by oleaginous microorganisms have attracted increasing interests as a petroleum alternative energy. Marine eukaryotes, thraustochytrids were heterotrophic, and can grow rapidly and accumulate large amount of lipids containing functional fatty acids, such as docosahexaenoic acid (DHA) in their cells body. In this investigation, thraustochytrids isolated from marine environment were cultured in the medium containing an industrial waste and an unused resource, distillery lees (Shochu kasu) to produce biofuel or functional fatty acids by microorganisms. Sixty-nine thraustochytrids and Schizochytrium aggregatum ATCC 28209 were screened for cellulase production, and the activities were detected using sodium carboxymethyl cellulose (CMC) as a substrate. Based on the screening test, strain TM02Bc identified to Schizochytrium sp. was selected for the Shochu kasu degradation test and compared with S. aggregatum ATCC 28209 previously known as a cellulase-producing thraustochytrid. Strains TM02Bc and ATCC 28209 were cultured in artificial seawater containing Shochu kasu for 15 days. The two strains could degrade Schochu kasu, especially that from sweet potato Shochu (Imo Shochu). Cellulase (CMCase) and protease activities were detected in culture supernatant of both strains, and the ratio of polyunsaturated fatty acids (PUFAs) significantly increased as a result of incubation of Shochu kasu with two strains. This preliminary study indicated that strain TM02Bc was a potent candidate for Shochu kasu treatment and fatty acid production.

元の言語英語
ページ(範囲)31-40
ページ数10
ジャーナルJournal of oleo science
66
発行部数1
DOI
出版物ステータス出版済み - 1 1 2017

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Distilleries
Cellulase
Fatty acids
Degradation
Fatty Acids
Microorganisms
Polyunsaturated fatty acids
Industrial Waste
Carboxymethylcellulose Sodium
Biofuels
Docosahexaenoic Acids
Industrial wastes
Petroleum
Unsaturated Fatty Acids
Seawater
Lipids
Cellulose
Screening
Oils
Peptide Hydrolases

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Chemical Engineering(all)

これを引用

Degradation of distillery lees (Shochu kasu) by cellulase-producing thraustochytrids. / Taoka, Yousuke; Nagano, Naoki; Kai, Hiroyuki; Hayashi, Masahiro.

:: Journal of oleo science, 巻 66, 番号 1, 01.01.2017, p. 31-40.

研究成果: ジャーナルへの寄稿記事

Taoka, Yousuke ; Nagano, Naoki ; Kai, Hiroyuki ; Hayashi, Masahiro. / Degradation of distillery lees (Shochu kasu) by cellulase-producing thraustochytrids. :: Journal of oleo science. 2017 ; 巻 66, 番号 1. pp. 31-40.
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