Deletion of epithelial cell-specific p130Cas impairs the maturation stage of amelogenesis

Akane Inoue, Tamotsu Kiyoshima, Keigo Yoshizaki, Chihiro Nakatomi, Mitsushiro Nakatomi, Hayato Ohshima, Masashi Shin, Jing Gao, Kanji Tsuru, Koji Okabe, Ichiro Nakamura, Hiroaki Honda, Miho Matsuda, Ichiro Takahashi, Eijiro Jimi

研究成果: Contribution to journalArticle査読

抄録

Amelogenesis consists of secretory, transition, maturation, and post-maturation stages, and the morphological changes of ameloblasts at each stage are closely related to their function. p130 Crk-associated substrate (Cas) is a scaffold protein that modulates essential cellular processes, including cell adhesion, cytoskeletal changes, and polarization. The expression of p130Cas was observed from the secretory stage to the maturation stage in ameloblasts. Epithelial cell-specific p130Cas-deficient (p130CasΔepi-) mice exhibited enamel hypomineralization with chalk-like white mandibular incisors in young mice and attrition in aged mouse molars. A micro-computed tomography analysis and Vickers micro-hardness testing showed thinner enamel, lower enamel mineral density and hardness in p130CasΔepi- mice in comparison to p130Casflox/flox mice. Scanning electron microscopy, and an energy dispersive X-ray spectroscopy analysis indicated the disturbance of the enamel rod structure and lower Ca and P contents in p130CasΔepi- mice, respectively. The disorganized arrangement of ameloblasts, especially in the maturation stage, was observed in p130CasΔepi- mice. Furthermore, expression levels of enamel matrix proteins, such as amelogenin and ameloblastin in the secretory stage, and functional markers, such as alkaline phosphatase and iron accumulation, and Na+/Ca2++K+-exchanger in the maturation stage were reduced in p130CasΔepi- mice. These findings suggest that p130Cas plays important roles in amelogenesis (197 words).

本文言語英語
論文番号116210
ジャーナルBone
154
DOI
出版ステータス出版済み - 1 2022

All Science Journal Classification (ASJC) codes

  • 内分泌学、糖尿病および代謝内科学
  • 組織学
  • 生理学

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