Demethylation by 5‐Azacytidine Results in the Expression of Hepatitis B Virus Surface Antigen in Transgenic Mice

Kimi Araki, Jun‐ichi ‐i Miyazaki, Toshiki Tsurimoto, Takeaki Inomoto, Tomohisa Iwanaga, Kenichi Matsubara, Ken‐ichi ‐i Yamamura

研究成果: ジャーナルへの寄稿記事

18 引用 (Scopus)

抄録

In 14p3HB transgenic mice, which carry three tandem copies of hepatitis B virus (HBV) DNA, the HBV DNA was significantly methylated and no viral proteins were produced. To analyze the causal relationship between hypermethylation and gene inactivity, 5‐azacytidine was injected into the mice to demethylate HBV DNA. When postnatal 14p3HB mice were treated with the drug, hepatitis virus surface antigen was produced in these mice by 3 weeks of age, and the integrated HBV DNA of the liver was less heavily methylated. Our results suggest that injection of 5‐azacytidine can be used to efficiently activate a silent transgene such as HBV DNA in transgenic mice.

元の言語英語
ページ(範囲)295-298
ページ数4
ジャーナルJapanese Journal of Cancer Research
80
発行部数4
DOI
出版物ステータス出版済み - 4 1989

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Hepatitis B Surface Antigens
Hepatitis B virus
Transgenic Mice
DNA
Hepatitis Viruses
Viral Proteins
Surface Antigens
Transgenes
Injections
Liver
Pharmaceutical Preparations
Genes

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

これを引用

Demethylation by 5‐Azacytidine Results in the Expression of Hepatitis B Virus Surface Antigen in Transgenic Mice. / Araki, Kimi; Miyazaki, Jun‐ichi ‐i; Tsurimoto, Toshiki; Inomoto, Takeaki; Iwanaga, Tomohisa; Matsubara, Kenichi; Yamamura, Ken‐ichi ‐i.

:: Japanese Journal of Cancer Research, 巻 80, 番号 4, 04.1989, p. 295-298.

研究成果: ジャーナルへの寄稿記事

Araki, Kimi ; Miyazaki, Jun‐ichi ‐i ; Tsurimoto, Toshiki ; Inomoto, Takeaki ; Iwanaga, Tomohisa ; Matsubara, Kenichi ; Yamamura, Ken‐ichi ‐i. / Demethylation by 5‐Azacytidine Results in the Expression of Hepatitis B Virus Surface Antigen in Transgenic Mice. :: Japanese Journal of Cancer Research. 1989 ; 巻 80, 番号 4. pp. 295-298.
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abstract = "In 14p3HB transgenic mice, which carry three tandem copies of hepatitis B virus (HBV) DNA, the HBV DNA was significantly methylated and no viral proteins were produced. To analyze the causal relationship between hypermethylation and gene inactivity, 5‐azacytidine was injected into the mice to demethylate HBV DNA. When postnatal 14p3HB mice were treated with the drug, hepatitis virus surface antigen was produced in these mice by 3 weeks of age, and the integrated HBV DNA of the liver was less heavily methylated. Our results suggest that injection of 5‐azacytidine can be used to efficiently activate a silent transgene such as HBV DNA in transgenic mice.",
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