Denaturing gradient gel electrophoresis analysis of gut bacterial community for the Japanese earthworms

Kazunori Nakamura, Kenji Sakai

研究成果: Contribution to journalArticle査読

2 被引用数 (Scopus)

抄録

The gut bacterial community structure for Pheretima hilgendorfi and P. heteropoda (Family Megascolecidae), and Allolobophora japonica (Family Lumbricidae) collected from agricultural grasslands in Japan was analyzed by denaturing gradient gel electrophoresis of polymerase chain reaction-amplified 16S rRNA gene fragments (PCR-DGGE) and compared with those in the surrounding soils. Denaturing gradient gel electrophoresis (DGGE) profiles indicated that each earthworm species had their own specific bacterial communities, and multidimentional scaling analysis grouped the DGGE profiles into three groups: gut samples from P. hilgendorfi and P. heteropoda, gut samples from A. japonica and samples from the surrounding soils. Nine dominant bands were identified by their direct sequencing and cloning. Major three bands from P. hilgendorfi and P. heteropoda were closely related to Bacillus species belonging to the phylum Firmicutes. Major four and two bands from A. japonica were closely related to the phyla Proteobacteria and Bacteroidetes, respectively.

本文言語英語
ページ(範囲)519-528
ページ数10
ジャーナルSoil Science and Plant Nutrition
57
4
DOI
出版ステータス出版済み - 12 1 2011

All Science Journal Classification (ASJC) codes

  • Soil Science
  • Plant Science

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