Dendritic sugar-microarrays by click chemistry

Tomohiro Fukuda, Shunsuke Onogi, Yoshiko Miura

研究成果: ジャーナルへの寄稿学術誌査読

41 被引用数 (Scopus)

抄録

Saccharide microarrays with well-defined multivalency were prepared using glycodendrimers. The glycodendrimers with various generations and saccharides (α-Man, β-GlcNAc and β-Gal) were synthesized and immobilized on an acetylenyl-terminated gold substrate via click chemistry. The saccharide-immobilized substrates were analyzed using XPS, ellipsometry, cyclic voltammetry, contact angle goniometry, QCM and FTIR-RAS. The biological abilities of the saccharide-immobilized substrates were evaluated by SPR with the lectins of Con A, WGA and RCA120. Lectin binding to the glycodendrimer array was quantitatively analyzed, and association constants (KA) and kinetic rate constants (ka and kd) were evaluated. These kinetic constants were unique to each lectin, implying specificity of the multivalent effect of saccharide-lectin interaction.

本文言語英語
ページ(範囲)880-888
ページ数9
ジャーナルThin Solid Films
518
2
DOI
出版ステータス出版済み - 11月 30 2009
外部発表はい

!!!All Science Journal Classification (ASJC) codes

  • 電子材料、光学材料、および磁性材料
  • 表面および界面
  • 表面、皮膜および薄膜
  • 金属および合金
  • 材料化学

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