Deregulation of nicotinamide N-methyltransferase and gap junction protein alpha-1 causes metastasis in adenoid cystic carcinoma

Kana Ishibashi, kotaro ishii, Goro Sugiyama, Tomoki Sumida, Tsuyoshi Sugiura, Yu Kamata, Katsuhiro Seki, Takahiro Fujinaga, Kumamaru Wataru, Yosuke Kobayashi, Naomi Hiyake, Hiroyuki Nakano, Tomohiro Yamada, Yoshihide Mori

研究成果: ジャーナルへの寄稿記事

1 引用 (Scopus)

抄録

Background/Aim: Adenoid cystic carcinoma (AdCC) is a malignant tumor that occurs in the salivary glands and frequently metastasizes. The aim of this study was to identify factors mediating AdCC metastasis. Materials and Methods: We established three AdCC cell lines by orthotropic transplantation and in vivo selection: parental, highly metastatic (ACCS-M-GFP), and lymph node metastatic (ACCS-LN-GFP) cells. Results: We examined the three cell lines. DNA microarray indicated significantly altered processes in ACCS-LN-GFP cells: particularly, the expression of nicotinamide N-methyltransferase (NNMT) was enhanced the most. NNMT is associated with tumorigenesis and is a potential tumor biomarker. Concomitantly, we found-significant down-regulation of gap junction protein alpha-1. We suggest that ACCS-LN-GFP cells acquire cancer stem cell features involving the up-regulation of NNMT and the loss of gap junction protein alpha-1, leading to epithelial-mesenchymal transition and consequent AdCC metastasis. Conclusion: NNMT is a potential biomarker of AdCC.

元の言語英語
ページ(範囲)187-197
ページ数11
ジャーナルAnticancer Research
38
発行部数1
DOI
出版物ステータス出版済み - 1 1 2018

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Nicotinamide N-Methyltransferase
Adenoid Cystic Carcinoma
Connexins
Neoplasm Metastasis
Cell Line
Epithelial-Mesenchymal Transition
Neoplastic Stem Cells
Tumor Biomarkers
Salivary Glands
Oligonucleotide Array Sequence Analysis
Carcinogenesis
Up-Regulation
Down-Regulation
Transplantation
Biomarkers
Lymph Nodes

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

これを引用

Deregulation of nicotinamide N-methyltransferase and gap junction protein alpha-1 causes metastasis in adenoid cystic carcinoma. / Ishibashi, Kana; ishii, kotaro; Sugiyama, Goro; Sumida, Tomoki; Sugiura, Tsuyoshi; Kamata, Yu; Seki, Katsuhiro; Fujinaga, Takahiro; Wataru, Kumamaru; Kobayashi, Yosuke; Hiyake, Naomi; Nakano, Hiroyuki; Yamada, Tomohiro; Mori, Yoshihide.

:: Anticancer Research, 巻 38, 番号 1, 01.01.2018, p. 187-197.

研究成果: ジャーナルへの寄稿記事

Ishibashi, Kana ; ishii, kotaro ; Sugiyama, Goro ; Sumida, Tomoki ; Sugiura, Tsuyoshi ; Kamata, Yu ; Seki, Katsuhiro ; Fujinaga, Takahiro ; Wataru, Kumamaru ; Kobayashi, Yosuke ; Hiyake, Naomi ; Nakano, Hiroyuki ; Yamada, Tomohiro ; Mori, Yoshihide. / Deregulation of nicotinamide N-methyltransferase and gap junction protein alpha-1 causes metastasis in adenoid cystic carcinoma. :: Anticancer Research. 2018 ; 巻 38, 番号 1. pp. 187-197.
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abstract = "Background/Aim: Adenoid cystic carcinoma (AdCC) is a malignant tumor that occurs in the salivary glands and frequently metastasizes. The aim of this study was to identify factors mediating AdCC metastasis. Materials and Methods: We established three AdCC cell lines by orthotropic transplantation and in vivo selection: parental, highly metastatic (ACCS-M-GFP), and lymph node metastatic (ACCS-LN-GFP) cells. Results: We examined the three cell lines. DNA microarray indicated significantly altered processes in ACCS-LN-GFP cells: particularly, the expression of nicotinamide N-methyltransferase (NNMT) was enhanced the most. NNMT is associated with tumorigenesis and is a potential tumor biomarker. Concomitantly, we found-significant down-regulation of gap junction protein alpha-1. We suggest that ACCS-LN-GFP cells acquire cancer stem cell features involving the up-regulation of NNMT and the loss of gap junction protein alpha-1, leading to epithelial-mesenchymal transition and consequent AdCC metastasis. Conclusion: NNMT is a potential biomarker of AdCC.",
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AU - Ishibashi, Kana

AU - ishii, kotaro

AU - Sugiyama, Goro

AU - Sumida, Tomoki

AU - Sugiura, Tsuyoshi

AU - Kamata, Yu

AU - Seki, Katsuhiro

AU - Fujinaga, Takahiro

AU - Wataru, Kumamaru

AU - Kobayashi, Yosuke

AU - Hiyake, Naomi

AU - Nakano, Hiroyuki

AU - Yamada, Tomohiro

AU - Mori, Yoshihide

PY - 2018/1/1

Y1 - 2018/1/1

N2 - Background/Aim: Adenoid cystic carcinoma (AdCC) is a malignant tumor that occurs in the salivary glands and frequently metastasizes. The aim of this study was to identify factors mediating AdCC metastasis. Materials and Methods: We established three AdCC cell lines by orthotropic transplantation and in vivo selection: parental, highly metastatic (ACCS-M-GFP), and lymph node metastatic (ACCS-LN-GFP) cells. Results: We examined the three cell lines. DNA microarray indicated significantly altered processes in ACCS-LN-GFP cells: particularly, the expression of nicotinamide N-methyltransferase (NNMT) was enhanced the most. NNMT is associated with tumorigenesis and is a potential tumor biomarker. Concomitantly, we found-significant down-regulation of gap junction protein alpha-1. We suggest that ACCS-LN-GFP cells acquire cancer stem cell features involving the up-regulation of NNMT and the loss of gap junction protein alpha-1, leading to epithelial-mesenchymal transition and consequent AdCC metastasis. Conclusion: NNMT is a potential biomarker of AdCC.

AB - Background/Aim: Adenoid cystic carcinoma (AdCC) is a malignant tumor that occurs in the salivary glands and frequently metastasizes. The aim of this study was to identify factors mediating AdCC metastasis. Materials and Methods: We established three AdCC cell lines by orthotropic transplantation and in vivo selection: parental, highly metastatic (ACCS-M-GFP), and lymph node metastatic (ACCS-LN-GFP) cells. Results: We examined the three cell lines. DNA microarray indicated significantly altered processes in ACCS-LN-GFP cells: particularly, the expression of nicotinamide N-methyltransferase (NNMT) was enhanced the most. NNMT is associated with tumorigenesis and is a potential tumor biomarker. Concomitantly, we found-significant down-regulation of gap junction protein alpha-1. We suggest that ACCS-LN-GFP cells acquire cancer stem cell features involving the up-regulation of NNMT and the loss of gap junction protein alpha-1, leading to epithelial-mesenchymal transition and consequent AdCC metastasis. Conclusion: NNMT is a potential biomarker of AdCC.

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