Design and analysis of a structural RNA that acts as a template for peptide ligation.

Norimasa Kashiwagi, Hiroyuki Furuta, Yoshiya Ikawa

    研究成果: Contribution to journalArticle査読

    3 被引用数 (Scopus)

    抄録

    An artificial RNA, which acts as a specific template for peptide ligation, was designed and constructed. Into the P4-P6 domain of the Tetrahymena intron RNA, two peptide binding motifs (boxB and RRE) were installed. Two peptides (N and Rev) were expected to bind to the RNA simultaneously, facilitating their ligation by an entropic effect. The peptide ligation actually proceeded effectively in the presence of the designer RNA. Analysis using mutant peptides or mutant RNAs demonstrated that the peptide ligation proceeded by forming a specific trimolecular RNP complex.

    本文言語英語
    ページ(範囲)387-388
    ページ数2
    ジャーナルNucleic acids symposium series (2004)
    51
    DOI
    出版ステータス出版済み - 2007

    All Science Journal Classification (ASJC) codes

    • Medicine(all)

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