抄録
A semi-confocal fluorescence microscope is designed and constructed aiming to observe fluorescence excitation spectrum of soluble molecules adsorbed at the air/water interface. With a conventional confocal fluorescence microscope, the target molecules of pyrene arises some difficulties: excitation wavelength in deep ultraviolet forces low transmission performance of microscopic objectives and low wavelength-tunable performance of an excitation laser. A total internal reflection illumination with a light-guide coupled monochromated-xenon lamp is combined with the confocal fluorescence microscope. Theoretical feasibility and performance of the microscope are discussed with preliminary results obtained.
本文言語 | 英語 |
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ページ(範囲) | 1-4 |
ページ数 | 4 |
ジャーナル | Evergreen |
巻 | 2 |
号 | 2 |
DOI | |
出版ステータス | 出版済み - 2015 |
!!!All Science Journal Classification (ASJC) codes
- 電子材料、光学材料、および磁性材料
- セラミックおよび複合材料
- 表面、皮膜および薄膜
- マネジメント、モニタリング、政策と法律