Design of RT-PCR primers method considered by gene family

Ken Ichi Makino, Kenta Tomonaga, Hideyasu Hirano, Masahiro Nakano, Tadahiro Kin, Hlroyuki Matsuura, Kazuharu Koide, Nobuo Noda

研究成果: Contribution to journalArticle査読

10 被引用数 (Scopus)

抄録

TO detect mRNA expression levels were very useful not only for the biological studies but also for the progress of medical therapy. Furthermore, mRNA transcription suppression caused by antisense also clearized and made possible the speedy result of the cell biological investigation. We created primer design protocol using fuzzy deducing theory, because most of the traditional computer protocol succeeded less than 20%. 90% of Our primers functioned after following checking (1. GC Contents Check; 8. Self Ho-mology Check; 3. Fuzzy Deducing Homologous Check: [versus a.: GAPDH, b: Profüin, c: β-actin]). We also added 4: gene family checking to target into a real mRNA out of similar sequences, and 5; 5'-, & -3' homology checking. Fuzzy deducing checking protocol made it possible to select real VEGF mRNA from a gene family in which there are many similar seauences. ICIC International

本文言語英語
ページ(範囲)269-274
ページ数6
ジャーナルInternational Journal of Innovative Computing, Information and Control
4
2
出版ステータス出版済み - 2 1 2008

All Science Journal Classification (ASJC) codes

  • ソフトウェア
  • 理論的コンピュータサイエンス
  • 情報システム
  • 計算理論と計算数学

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