Design of UCST polymers for chilling capture of proteins

Naohiko Shimada, Miki Nakayama, Arihiro Kano, Atsushi Maruyama

研究成果: ジャーナルへの寄稿学術誌査読

72 被引用数 (Scopus)


Ureido-derivatized polymers, such as poly(allylurea) (PU) and poly( L-citrulline) derivatives, exhibited upper critical solution temperature (UCST) behavior under physiological buffer conditions as we previously reported. The PU derivatives having amino groups (PU-Am) also showed UCST behavior. In this study, we modified the amino groups of the polymer with succinyl anhydride (PU-Su) or acetyl anhydride (PU-Ac) to determine the effects of these ionic groups on the UCST behavior and to control interactions between the PU derivatives and biocomponents such as proteins and cells. Succinylation of PU-Am resulted in a significant decrease in phase separation temperature (Tp), whereas acetylation of PU-Am resulted in an increase in T p. As expected, the Tp of PU-Am and PU-Su changed when the pH of the solution was changed. The Tp of PU-Am increased at higher pH, whereas that of PU-Su increased at lower pH, indicating that ionic charge decreases Tp of PU derivatives by increasing osmotic pressure and by increasing hydrophilicity of the polymer chains. Interestingly, these groups did not significantly change UCST when these groups were nonionic. We then examined capture and separation of particular proteins from a protein mixture by cooling-induced phase separation. Selective and rapid capture of particular proteins from protein mixture by PU derivatives was shown, indicating that the ureido-derivatized polymers are potential media for bioseparation under biofriendly conditions.

出版ステータス出版済み - 5月 13 2013

!!!All Science Journal Classification (ASJC) codes

  • バイオエンジニアリング
  • 生体材料
  • ポリマーおよびプラスチック
  • 材料化学


「Design of UCST polymers for chilling capture of proteins」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。