Detecting protein – DNA interactions using a modified yeast one-hybrid system

Kazuhisa Ota, Shu Ying Feng, Takashi Ito

研究成果: Contribution to journalArticle査読

3 被引用数 (Scopus)

抄録

The yeast one-hybrid (Y1H) system has been among the methods of choice to detect protein–DNA interactions. However, conventional Y1H systems with a single auxotrophic reporter gene often suffer from high incidence of false positives to demonstrate a limited power in large-scale screenings. Here we describe a refined Y1H system that uses two independent bait sequences, each controlling a distinct reporter gene integrated in the host genome. With these modifications and a method of targeted DNA methylation, we succeeded in efficient isolation of clones for methylated DNA-binding proteins from mammalian cDNA libraries.

本文言語英語
ページ(範囲)39-50
ページ数12
ジャーナルMethods in Molecular Biology
1164
DOI
出版ステータス出版済み - 2014

All Science Journal Classification (ASJC) codes

  • 分子生物学
  • 遺伝学

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