Detecting protein – DNA interactions using a modified yeast one-hybrid system

Kazuhisa Ota, Shu Ying Feng, Takashi Ito

研究成果: ジャーナルへの寄稿記事

3 引用 (Scopus)

抄録

The yeast one-hybrid (Y1H) system has been among the methods of choice to detect protein–DNA interactions. However, conventional Y1H systems with a single auxotrophic reporter gene often suffer from high incidence of false positives to demonstrate a limited power in large-scale screenings. Here we describe a refined Y1H system that uses two independent bait sequences, each controlling a distinct reporter gene integrated in the host genome. With these modifications and a method of targeted DNA methylation, we succeeded in efficient isolation of clones for methylated DNA-binding proteins from mammalian cDNA libraries.

元の言語英語
ページ(範囲)39-50
ページ数12
ジャーナルMethods in Molecular Biology
1164
DOI
出版物ステータス出版済み - 1 1 2014

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Reporter Genes
Yeasts
DNA
DNA-Binding Proteins
DNA Methylation
Gene Library
Proteins
Clone Cells
Genome
Incidence

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

これを引用

Detecting protein – DNA interactions using a modified yeast one-hybrid system. / Ota, Kazuhisa; Feng, Shu Ying; Ito, Takashi.

:: Methods in Molecular Biology, 巻 1164, 01.01.2014, p. 39-50.

研究成果: ジャーナルへの寄稿記事

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