Detection and inhibition of lipid-derived radicals in low-density lipoprotein

Yuma Ishida, Yuka Okamoto, Yuta Matsuoka, Arisa Tada, Jindaporn Janprasit, Mayumi Yamato, Noppawan Phumala Morales, Ken-Ichi Yamada

研究成果: ジャーナルへの寄稿記事

2 引用 (Scopus)

抄録

Oxidized low density lipoprotein (Ox-LDL) is implicated in a variety of oxidative diseases. To clarify the mechanisms involved and facilitate the investigation of therapeutics, we previously developed a detection method for lipid-derived radicals using the fluorescent probe 2,2,6-trimethyl-6-pentyl-4-(4-nitrobenzo[1,2,5]oxadiazol-7-ylamino)piperidine-1-oxyl (NBD-Pen). In this study, NBD-Pen was used to detect lipid-derived radicals in Ox-LDL from in vitro and in vivo samples using an iron overloaded mouse model. By following the timeline of lipid radical generation using this method, the iron overloaded mice could be successfully treated with the antioxidant Trolox, resulting in successful lowering of the plasma lipid peroxidation, aspartate transaminase and alanine transaminase levels. Furthermore, using a combination therapy of the chelating agent deferoxamine (DFX) and Trolox, liver injury and oxidative stress markers were also reduced in iron overloaded mice. The NBD-Pen method is highly sensitive as well as selective and is suitable for targeting minimally modified LDL compared with other existing methods.

元の言語英語
ページ(範囲)487-493
ページ数7
ジャーナルFree Radical Biology and Medicine
113
DOI
出版物ステータス出版済み - 12 1 2017

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LDL Lipoproteins
Lipids
Iron
Deferoxamine
Oxidative stress
Chelating Agents
Aspartate Aminotransferases
Alanine Transaminase
Fluorescent Dyes
Liver
Lipid Peroxidation
Oxidative Stress
Antioxidants
Plasmas
Wounds and Injuries
Therapeutics
oxidized low density lipoprotein
6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Physiology (medical)

これを引用

Detection and inhibition of lipid-derived radicals in low-density lipoprotein. / Ishida, Yuma; Okamoto, Yuka; Matsuoka, Yuta; Tada, Arisa; Janprasit, Jindaporn; Yamato, Mayumi; Morales, Noppawan Phumala; Yamada, Ken-Ichi.

:: Free Radical Biology and Medicine, 巻 113, 01.12.2017, p. 487-493.

研究成果: ジャーナルへの寄稿記事

Ishida, Yuma ; Okamoto, Yuka ; Matsuoka, Yuta ; Tada, Arisa ; Janprasit, Jindaporn ; Yamato, Mayumi ; Morales, Noppawan Phumala ; Yamada, Ken-Ichi. / Detection and inhibition of lipid-derived radicals in low-density lipoprotein. :: Free Radical Biology and Medicine. 2017 ; 巻 113. pp. 487-493.
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