Detection of bidirectional promoter-derived lncRNAs from small-scale samples using pre-amplification-free directional RNA-seq method

研究成果: 著書/レポートタイプへの貢献

2 引用 (Scopus)

抄録

Development of high-throughput sequencing technologies has uncovered the immensity of the long noncoding RNA (lncRNA) world. Divergently transcribed lncRNAs from bidirectional gene promoters, called promoter-associated noncoding RNAs (pancRNAs), account for ~20% of the total number of lncRNAs, and this major fraction is involved in many biological processes, such as development and cancer formation. Recently, we have found that the pancRNAs activate their partner genes, as represented by the fact that pancIl17d, a pancRNA that is transcribed from the antisense strand of the promoter region of Interleukin 17d (Il17d) at the onset of zygotic gene activation (ZGA), is essential for mouse preimplantation development through Il17d upregulation. The discovery of the expression of a specific set of pancRNAs during ZGA was achieved by using a method that generates directional RNA-seq libraries from small-scale samples. Although there are several methods available for small-scale samples, most of them require a pre-amplification procedure that frequently generates some amplification biases toward a subset of transcripts. We provide here a highly sensitive and reproducible method based on the preparation of directional RNA-seq libraries from as little as 100 mouse oocytes or embryos without pre-amplification for the quantification of lncRNAs as well as mRNAs.

元の言語英語
ホスト出版物のタイトルMethods in Molecular Biology
出版者Humana Press Inc.
ページ83-103
ページ数21
DOI
出版物ステータス出版済み - 1 1 2017

出版物シリーズ

名前Methods in Molecular Biology
1605
ISSN(印刷物)1064-3745

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Long Noncoding RNA
Untranslated RNA
Interleukin-27
RNA
Transcriptional Activation
Libraries
Biological Phenomena
Genetic Promoter Regions
Genes
Oocytes
Up-Regulation
Embryonic Structures
Technology
Messenger RNA
Neoplasms

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

これを引用

Detection of bidirectional promoter-derived lncRNAs from small-scale samples using pre-amplification-free directional RNA-seq method. / Hamazaki, Nobuhiko; Nakashima, Kinichi; Hayashi, Katsuhiko; Imamura, Takuya.

Methods in Molecular Biology. Humana Press Inc., 2017. p. 83-103 (Methods in Molecular Biology; 巻 1605).

研究成果: 著書/レポートタイプへの貢献

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