表面プラズモン共鳴センサを用いた尿中カテコールアミン代謝物の検出

Takeshi Onodera, Takuya Eda, Hideto Sonoda, Toshiro Matsui, Kiyoshi Toko

研究成果: Contribution to journalArticle査読

抄録

In this study, an Au sensor surface of surface plasmon resonance was developed for detection of catecholamine metabolites such as homovanilic acid (HVA) in urine samples. The sensor surface was modified with mixed self-assembled monolayer containing oligo (ethylene glycol) chain for reducing nonspecific adsorption. An indirect competitive assay using anti-HVA-concholepas concholepas hemocyanin (CCH) polyclonal antibody and HVA in model of urine solution was carried out. HVA concentrations of human urine samples were estimated from SPR sensor responses by referring the response curve. As the results, the detection limit of HVA in model of urine was obtained 15 ppb. The rank of estimated concentration in five human urine samples was matched with that of the quantified concentration by high performance liquid chromatography-mass spectrometry.

寄稿の翻訳タイトルDetection of catecholamine metabolite in urine using surface plasmon resonance sensor
本文言語日本語
ページ(範囲)278-282
ページ数5
ジャーナルIEEJ Transactions on Sensors and Micromachines
139
9
DOI
出版ステータス出版済み - 1 1 2019

All Science Journal Classification (ASJC) codes

  • 機械工学
  • 電子工学および電気工学

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