Detection of cry-type gene homologues in the draft genome sequence of paenibacillus popilliae ATCC 14706t and trial for the expression of recombinant proteins in escherichia coli

Akio Kawahara, Kazuhiro Iiyama, Shin Ichiro Asano, Oumi Nishi, Chisa Yasunaga-Aoki

研究成果: Contribution to journalArticle査読

抄録

Two novel cry-type gene homologues, tentatively named cryPp1 and cryPp2, were detected in the genomic DNA of Paenibacillus popilliae ATCC 14706T, both of which were similar to genes encoding Cry18 proteins, suggesting their similar modes of action. Ours is the first report concerning multiple Cry homologues, detected from a sole strain of P. popilliae. In order to characterize the roles of the two Cry homologues in pathogenicity, a trial for the expression of recombinant proteins in Escherichia coli was conducted, which revealed that the optimization of rare codons, such as supplementation of tRNA genes, was necessary.

本文言語英語
ページ(範囲)25-30
ページ数6
ジャーナルJournal of Insect Biotechnology and Sericology
89
2
DOI
出版ステータス出版済み - 2020

All Science Journal Classification (ASJC) codes

  • バイオテクノロジー
  • 応用微生物学とバイオテクノロジー
  • ビジネス、管理および会計(全般)
  • 農業および生物科学(全般)
  • 昆虫科学
  • 産業および生産工学

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