Detection of cry-type gene homologues in the draft genome sequence of paenibacillus popilliae ATCC 14706t and trial for the expression of recombinant proteins in escherichia coli

Akio Kawahara; Kazuhiro Iiyama; Shin Ichiro Asano; Oumi Nishi; Chisa Yasunaga-Aoki

doi:10.11416/jibs.89.2_025

Detection of cry-type gene homologues in the draft genome sequence of paenibacillus popilliae ATCC 14706^t and trial for the expression of recombinant proteins in escherichia coli

Akio Kawahara, Kazuhiro Iiyama, Shin Ichiro Asano, Oumi Nishi, Chisa Yasunaga-Aoki

農業生物科学

研究成果: ジャーナルへの寄稿 › 学術誌 › 査読

抄録

Two novel cry-type gene homologues, tentatively named cryPp1 and cryPp2, were detected in the genomic DNA of Paenibacillus popilliae ATCC 14706^T, both of which were similar to genes encoding Cry18 proteins, suggesting their similar modes of action. Ours is the first report concerning multiple Cry homologues, detected from a sole strain of P. popilliae. In order to characterize the roles of the two Cry homologues in pathogenicity, a trial for the expression of recombinant proteins in Escherichia coli was conducted, which revealed that the optimization of rare codons, such as supplementation of tRNA genes, was necessary.

本文言語	英語
ページ（範囲）	25-30
ページ数	6
ジャーナル	Journal of Insect Biotechnology and Sericology
巻	89
号	2
DOI	https://doi.org/10.11416/jibs.89.2_025
出版ステータス	出版済み - 2020

!!!All Science Journal Classification (ASJC) codes

バイオテクノロジー
応用微生物学とバイオテクノロジー
ビジネス、管理および会計（全般）
農業および生物科学（全般）
昆虫科学
産業および生産工学

文献へのアクセス

10.11416/jibs.89.2_025

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引用スタイル

Detection of cry-type gene homologues in the draft genome sequence of paenibacillus popilliae ATCC 14706^t and trial for the expression of recombinant proteins in escherichia coli. / Kawahara, Akio; Iiyama, Kazuhiro; Asano, Shin Ichiro その他.
In: Journal of Insect Biotechnology and Sericology, Vol. 89, No. 2, 2020, p. 25-30.

研究成果: ジャーナルへの寄稿 › 学術誌 › 査読

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abstract = "Two novel cry-type gene homologues, tentatively named cryPp1 and cryPp2, were detected in the genomic DNA of Paenibacillus popilliae ATCC 14706T, both of which were similar to genes encoding Cry18 proteins, suggesting their similar modes of action. Ours is the first report concerning multiple Cry homologues, detected from a sole strain of P. popilliae. In order to characterize the roles of the two Cry homologues in pathogenicity, a trial for the expression of recombinant proteins in Escherichia coli was conducted, which revealed that the optimization of rare codons, such as supplementation of tRNA genes, was necessary.",

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