抄録
Ganoderma is a genus of medicinal mushroom traditionally used for treating various diseases. Ganoderic acid A is one of the major bioactive Ganoderma triterpenoids isolated from Ganoderma species. Herein, we produced a highly specific monoclonal antibody against ganoderic acid A (MAb 12 A) and developed an indirect competitive ELISA for the highly sensitive detection of ganoderic acid A in Ganoderma lingzhi, with a limit of detection of 6.10 ng/mL. Several validation analyses support the accuracy and reliability of the developed indirect competitive ELISA for use in the quality control of Ganoderma based on ganoderic acid A content. Furthermore, quantitative analysis of ganoderic acid A in G. lingzhi revealed that the pileus exhibits the highest ganoderic acid A content compared with the stipe and spore of the fruiting body; the best extraction efficiency was found when 50 % ethanol was used, which suggests the use of a strong liquor to completely harness the potential of Ganoderma triterpenoids in daily life.
本文言語 | 英語 |
---|---|
ページ(範囲) | 747-751 |
ページ数 | 5 |
ジャーナル | Planta medica |
巻 | 82 |
号 | 8 |
DOI | |
出版ステータス | 出版済み - 5月 1 2016 |
!!!All Science Journal Classification (ASJC) codes
- 分析化学
- 分子医療
- 薬理学
- 薬科学
- 創薬
- 補完代替医療
- 有機化学