TY - JOUR
T1 - Detection of pre-harvest sprouting in rice seeds by using1H-NMR
AU - Ishibashi, Yushi
AU - Sueyoshi, Reiko
AU - Morita, Tomoka
AU - Yoshimura, Atsushi
AU - Iwaya-Inoue, Mari
PY - 2005
Y1 - 2005
N2 - Pre-harvest sprouting (PHS) is a serious source of costly yield and grade losses for producers. Unreleased test lines of cereals should be screened for resistance to PHS. However, screening large numbers of test lines is relatively time-consuming or expensive. In this study, nuclear magnetic resonance (NMR) was used to characterize PHS as a nondestructive method. The water content in the rice grains of 'TC65' and 'Notched' gradually decreased until 21 days after pollination (DAP). After 21 DAP, the water content in both lines remained constant. In PHS at this time, none of the 'TC65' seeds germinated while 15% of the 'Notched' seeds had germinated. At 14 DAP when PHS was not confirmed, NMR spin-spin relaxation time (T2) in 'TC65' and 'Notched' seeds were about 40 ms and 20 ms, respectively. These results indicated that the T 2 is effective to screen resistance to PHS at early stage. In addition, T2s in germinating seeds linearly increased to 165 ms as germination advanced during 10-day treatment period. On the contrary, T 2s in nongerminating seeds were about 20 ms and they remained constant during the treatment period. Thus, 1H-NMR relaxation times were measured as a way to screen both resistance to PHS and germination process.
AB - Pre-harvest sprouting (PHS) is a serious source of costly yield and grade losses for producers. Unreleased test lines of cereals should be screened for resistance to PHS. However, screening large numbers of test lines is relatively time-consuming or expensive. In this study, nuclear magnetic resonance (NMR) was used to characterize PHS as a nondestructive method. The water content in the rice grains of 'TC65' and 'Notched' gradually decreased until 21 days after pollination (DAP). After 21 DAP, the water content in both lines remained constant. In PHS at this time, none of the 'TC65' seeds germinated while 15% of the 'Notched' seeds had germinated. At 14 DAP when PHS was not confirmed, NMR spin-spin relaxation time (T2) in 'TC65' and 'Notched' seeds were about 40 ms and 20 ms, respectively. These results indicated that the T 2 is effective to screen resistance to PHS at early stage. In addition, T2s in germinating seeds linearly increased to 165 ms as germination advanced during 10-day treatment period. On the contrary, T 2s in nongerminating seeds were about 20 ms and they remained constant during the treatment period. Thus, 1H-NMR relaxation times were measured as a way to screen both resistance to PHS and germination process.
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U2 - 10.2525/ecb.43.131
DO - 10.2525/ecb.43.131
M3 - Article
AN - SCOPUS:33646181099
VL - 43
SP - 131
EP - 137
JO - Environmental Control in Biology
JF - Environmental Control in Biology
SN - 1880-554X
IS - 2
ER -