Detection of SARS-CoV-2 Gene by Microbeads Dielectrophoresis-based DNA Detection Method

研究成果: Contribution to journalArticle査読

抄録

Nucleic amplification tests (NATs) are sensitive and specific methods to diagnose infectious diseases. Real-time polymerase chain reaction (PCR) is the gold standard, but it requires expensive apparatus and regents because of fluorescent detection. This study performed a combination of a conventional PCR and microbeads dielectrophoresis (DEP)-based DNA detection method to detect the SARS-CoV-2 gene quantitatively. SARS-CoV-2 is a virus causing COVID-19 and has caused the pandemic worldwide. In the microbeads DEP-based DNA detection method, the amplified DNA, amplicon, is attached to microbeads, the amplicon-labeled microbeads are detected by dielectrophoretic impedance measurement. As a result, the technique demonstrated that same sensitivity as that of real-time PCR. Our method enables the cheap diagnosis of infectious disease instead of expensive real-time PCR.

本文言語英語
ページ(範囲)233-236
ページ数4
ジャーナルIEEJ Transactions on Sensors and Micromachines
141
7
DOI
出版ステータス出版済み - 2021

All Science Journal Classification (ASJC) codes

  • 機械工学
  • 電子工学および電気工学

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