Detection of SNPs in fish DNA: Application of the fluorogenic ribonuclease protection (FRIP) assay for the authentication of food contents

Momoko Kitaoka, Nobuko Okamura, Hirofumi Ichinose, Masahiro Goto

研究成果: ジャーナルへの寄稿記事

7 引用 (Scopus)

抄録

The fluorogenic ribonuclease protection (FRIP) assay was used to detect single nucleotide polymorphisms (SNPs) in commercially produced fish products. By using fluorescence resonance energy transfer (FRET) between fluorophore and quencher labeled probes, the species-specific cleavage of sample RNA was detected by measuring the fluorescence intensity during the FRIP assay. We were able to discriminate raw and thermally processed eel and tuna species using the FRIP-based SNP detection method. Furthermore, the intensity of fluorescence was correlated with the mutant/wild-type ratio. These results suggest that the FRIP assay is a useful method for the in situ confirmation of labels of fishery foods during food production.

元の言語英語
ページ(範囲)6246-6251
ページ数6
ジャーナルJournal of Agricultural and Food Chemistry
56
発行部数15
DOI
出版物ステータス出版済み - 8 13 2008

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ribonucleases
Ribonucleases
Polymorphism
Fish
Authentication
single nucleotide polymorphism
Single Nucleotide Polymorphism
Assays
Fishes
Nucleotides
Food
DNA
assays
fluorescence
fish
Fish products
Fluorescence
RNA Cleavage
Fish Products
Tuna

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Agricultural and Biological Sciences(all)

これを引用

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abstract = "The fluorogenic ribonuclease protection (FRIP) assay was used to detect single nucleotide polymorphisms (SNPs) in commercially produced fish products. By using fluorescence resonance energy transfer (FRET) between fluorophore and quencher labeled probes, the species-specific cleavage of sample RNA was detected by measuring the fluorescence intensity during the FRIP assay. We were able to discriminate raw and thermally processed eel and tuna species using the FRIP-based SNP detection method. Furthermore, the intensity of fluorescence was correlated with the mutant/wild-type ratio. These results suggest that the FRIP assay is a useful method for the in situ confirmation of labels of fishery foods during food production.",
author = "Momoko Kitaoka and Nobuko Okamura and Hirofumi Ichinose and Masahiro Goto",
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T1 - Detection of SNPs in fish DNA

T2 - Application of the fluorogenic ribonuclease protection (FRIP) assay for the authentication of food contents

AU - Kitaoka, Momoko

AU - Okamura, Nobuko

AU - Ichinose, Hirofumi

AU - Goto, Masahiro

PY - 2008/8/13

Y1 - 2008/8/13

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AB - The fluorogenic ribonuclease protection (FRIP) assay was used to detect single nucleotide polymorphisms (SNPs) in commercially produced fish products. By using fluorescence resonance energy transfer (FRET) between fluorophore and quencher labeled probes, the species-specific cleavage of sample RNA was detected by measuring the fluorescence intensity during the FRIP assay. We were able to discriminate raw and thermally processed eel and tuna species using the FRIP-based SNP detection method. Furthermore, the intensity of fluorescence was correlated with the mutant/wild-type ratio. These results suggest that the FRIP assay is a useful method for the in situ confirmation of labels of fishery foods during food production.

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