Detection of SNPs in fish DNA: Application of the fluorogenic ribonuclease protection (FRIP) assay for the authentication of food contents

Momoko Kitaoka, Nobuko Okamura, Hmofumi Ichinose, Masahiro Goto

研究成果: Contribution to journalArticle査読

9 被引用数 (Scopus)

抄録

The fluorogenic ribonuclease protection (FRIP) assay was used to detect single nucleotide polymorphisms (SNPs) in commercially produced fish products. By using fluorescence resonance energy transfer (FRET) between fluorophore and quencher labeled probes, the species-specific cleavage of sample RNA was detected by measuring the fluorescence intensity during the FRIP assay. We were able to discriminate raw and thermally processed eel and tuna species using the FRIP-based SNP detection method. Furthermore, the intensity of fluorescence was correlated with the mutant/wild-type ratio. These results suggest that the FRIP assay is a useful method for the in situ confirmation of labels of fishery foods during food production.

本文言語英語
ページ(範囲)6246-6251
ページ数6
ジャーナルJournal of Agricultural and Food Chemistry
56
15
DOI
出版ステータス出版済み - 8 13 2008

All Science Journal Classification (ASJC) codes

  • 化学 (全般)
  • 農業および生物科学(全般)

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