Detection of the single-stranded DNA of Streptomyces plasmid pSA1.1 and a binding histone-like protein

Eiji Yokoyama, Katsumi Doi, Makoto Kimura, Seiya Ogata

研究成果: Contribution to journalArticle査読

7 被引用数 (Scopus)

抄録

Streptomyces plasmid pSA1.1 accumulated single-stranded DNA as replication intermediates in S. lividans; therefore, this plasmid was considered to replicate by a rolling-circle mechanism. A DNA-binding protein (pI > 9.7 and about 10 kDa) was purified on a denatured DNA-Cellulose column, then on a native DNA-Cellulose column. The N-terminal amino acid sequence of this protein has a high homology with bacterial histone-like proteins. In the gel retardation assay, this protein bound with the single-stranded DNA of pSA1.1. We propose that this protein may participate in the replication of pSA1.1.

本文言語英語
ページ(範囲)197-200
ページ数4
ジャーナルFEMS microbiology letters
138
2-3
DOI
出版ステータス出版済み - 5 1 1996

All Science Journal Classification (ASJC) codes

  • 微生物学
  • 分子生物学
  • 遺伝学

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