We devised a sensitive and simple method for determination of bromide in whole blood and urine from humans using gas chromatography-mass spectrometry. Bromide was alkylated with pentafluorobenzyl p-toluenesulphonate in the mixture of acetone and phosphate buffer (pH 6.8). The derivative obtained was analyzed using gas chromatography-mass spectrometry with the positive-ion EI mode. The lower limit of detection for the compound was 1 mg/l. The calibration curve for bromide was linear over the concentration range from 2 to 100 mg/l. With use of this method, levels of bromide in whole blood and urine were determined in cases of poisoning by inhaled brominated hydrocarbons.
|ジャーナル||Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences|
|出版ステータス||出版済み - 3 25 2005|
All Science Journal Classification (ASJC) codes
- Analytical Chemistry
- Clinical Biochemistry
- Cell Biology