TY - JOUR
T1 - Determination of curcumin in biologically active supplements and food spices using a mesofluidic platform with fluorescence detection
AU - Petrova, Anastasiia
AU - Dar'in, Dmitriy
AU - Ivanov, Aleхander
AU - Moskvin, Leonid
AU - Ishimatsu, Ryoichi
AU - Nakano, Koji
AU - Imato, Toshihiko
AU - Bulatov, Andrey
N1 - Funding Information:
New automated method was developed by financial supported from the Russian Scientific Foundation (project no. 16-13-10117 ). Andrey Bulatov gratefully acknowledges financial support for fluorometric investigations from the Russian Government (project no. MD-6597.2016.3 ) and the Russian Foundation for Basic Research (project no. 15-33-20068 ). Scientific research was partially performed at the Center for Chemical Analysis and Materials Research and the Research Centre for Magnetic Resonance of St. Petersburg State University.
PY - 2016/10/1
Y1 - 2016/10/1
N2 - A mesofluidic platform (MP) with fluorescence detection based on a stepwise injection analysis (SWIA) was used for the determination of curcumin in biologically active supplements and food spices. The main units of the MP are a mixing chamber (MC) and an optical channel with a quartz capillary inside. The MC provides rapid and complete mixing solutions by gas bubbling. The proposed method is based on the new rapid and sensitive reaction of curcumin with a fluorescence reagent – 4-(2,3,3-trimethyl-3H-indolium-1-yl)butane-1-sulfonate (TIBS). The fluorescence intensity of TIBS is greatly quenched in the presence of curcumin in an alkaline medium. The linear range was from 1 to 10 µM of curcumin, and the limit of detection, calculated as 3σ of a blank test (n=5), was found to be 0.3 µM. The sample throughput was 24 h−1. The proposed method was successfully applied for the determination of curcumin in biologically active supplements and samples of food spices. The obtained data were in good agreement with those measured by a HPLC-UV method.
AB - A mesofluidic platform (MP) with fluorescence detection based on a stepwise injection analysis (SWIA) was used for the determination of curcumin in biologically active supplements and food spices. The main units of the MP are a mixing chamber (MC) and an optical channel with a quartz capillary inside. The MC provides rapid and complete mixing solutions by gas bubbling. The proposed method is based on the new rapid and sensitive reaction of curcumin with a fluorescence reagent – 4-(2,3,3-trimethyl-3H-indolium-1-yl)butane-1-sulfonate (TIBS). The fluorescence intensity of TIBS is greatly quenched in the presence of curcumin in an alkaline medium. The linear range was from 1 to 10 µM of curcumin, and the limit of detection, calculated as 3σ of a blank test (n=5), was found to be 0.3 µM. The sample throughput was 24 h−1. The proposed method was successfully applied for the determination of curcumin in biologically active supplements and samples of food spices. The obtained data were in good agreement with those measured by a HPLC-UV method.
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U2 - 10.1016/j.talanta.2016.06.046
DO - 10.1016/j.talanta.2016.06.046
M3 - Article
C2 - 27474312
AN - SCOPUS:84978149821
VL - 159
SP - 300
EP - 306
JO - Talanta
JF - Talanta
SN - 0039-9140
ER -