Determination of guanine by high-performance liquid chromatography with electrochemical detection: Application to DNA and RNA assays

T. Nakahara, A. Shiraishi, M. Hirano, T. Matsumoto, T. Kuroki, Y. Tatebayashi, T. Tsutsumi, K. Nishiyama, H. Ooboshi, K. Nakamura, H. Yao, M. Waki, H. Uchimura

研究成果: Contribution to journalArticle査読

19 被引用数 (Scopus)

抄録

A highly sensitive assay for guanine was developed using high-performance liquid chromatography with electrochemical detection (ECD). Guanine was susceptible to the electrochemical oxidation, and ECD response was proportional to the amount of guanine in the range 0.25-4 pmol of guanine. The ECD of guanine was applicable to the analysis of nucleic acids. DNA and RNA were hydrolyzed in 0.03 and 3 M HCl, respectively, and guanine liberated from the nucleic acids was separated on a reverse-phase column and determined by ECD. The method allowed detection of 0.2 ng of calf thymus DNA or tRNA. An application of the method is shown for DNA and RNA assays in trichloroacetic acid extracts of rat adrenal and liver.

本文言語英語
ページ(範囲)38-42
ページ数5
ジャーナルAnalytical Biochemistry
180
1
出版ステータス出版済み - 1 1 1989

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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