Determination of hybrids in Papaver species by RAPD, HPLC and ELISA

Y. Shoyama, H. Tanaka, S. Morimoto, F. Taura

研究成果: Contribution to journalArticle査読

抄録

Total DNA was extracted from the leaves of a F1 hybrid and its parents, Papaver bracteatum and P. pseudo-orientale. Analysis of RAPD using ten arbitrary oligonucleotide 10-mers, showed that F1 hybrid was confirmed to be genetically intermediate of both parental plants compared with the genetic distance between F1 hybrid and individual parents. Furthermore, the comparison of the band patterns between a F1 hybrid, P. bracteatum and P. pseudo-orientale clearly showed that part of the bands of both parents were induced into a F1 hybrid. The content of thebaine was determined by HPLC and ELISA used anti-thebaine monoclonal antibody.

本文言語英語
ページ(範囲)160-161
ページ数2
ジャーナルJapanese Journal of Forensic Toxicology
18
2
出版ステータス出版済み - 2000

All Science Journal Classification (ASJC) codes

  • 病理学および法医学
  • 毒物学

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