Determination of hybrids in Papaver species by RAPD, HPLC and ELISA

Y. Shoyama, H. Tanaka, S. Morimoto, F. Taura

研究成果: ジャーナルへの寄稿記事

抄録

Total DNA was extracted from the leaves of a F1 hybrid and its parents, Papaver bracteatum and P. pseudo-orientale. Analysis of RAPD using ten arbitrary oligonucleotide 10-mers, showed that F1 hybrid was confirmed to be genetically intermediate of both parental plants compared with the genetic distance between F1 hybrid and individual parents. Furthermore, the comparison of the band patterns between a F1 hybrid, P. bracteatum and P. pseudo-orientale clearly showed that part of the bands of both parents were induced into a F1 hybrid. The content of thebaine was determined by HPLC and ELISA used anti-thebaine monoclonal antibody.

元の言語英語
ページ(範囲)160-161
ページ数2
ジャーナルJapanese Journal of Forensic Toxicology
18
発行部数2
出版物ステータス出版済み - 2000

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Thebaine
Papaver
Enzyme-Linked Immunosorbent Assay
High Pressure Liquid Chromatography
Oligonucleotides
Monoclonal Antibodies
DNA

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Toxicology

これを引用

Determination of hybrids in Papaver species by RAPD, HPLC and ELISA. / Shoyama, Y.; Tanaka, H.; Morimoto, S.; Taura, F.

:: Japanese Journal of Forensic Toxicology, 巻 18, 番号 2, 2000, p. 160-161.

研究成果: ジャーナルへの寄稿記事

Shoyama, Y. ; Tanaka, H. ; Morimoto, S. ; Taura, F. / Determination of hybrids in Papaver species by RAPD, HPLC and ELISA. :: Japanese Journal of Forensic Toxicology. 2000 ; 巻 18, 番号 2. pp. 160-161.
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