Determination of iriflophenone 3-c-β-d-glucoside from aquilaria spp. by an indirect competitive enzyme-linked immunosorbent assay using a specific polyclonal antibody

Waraporn Putalun, Gorawit Yusakul, Paritad Saensom, Boonchoo Sritularak, Hiroyuki Tanaka

研究成果: ジャーナルへの寄稿記事

3 引用 (Scopus)

抄録

Polyclonal antibody against iriflophenone 3-C-β-d-glucoside (IP3G), a major compound from the leaves of Aquilaria spp., was produced for the development of an enzyme-linked immunosorbent assay (ELISA). The results showed that the antibodies were specific for IP3G. The produced antibody has low cross reactivity with iriflophenone 3,5-C-β-d-diglucopyranoside (13%), genkwanin 5-O-β-primeveroside (3.55%) and no cross reactivity found in other compounds. The range of ELISA assay extends from 100 to 1560 ng/mL with coefficient of variation (CV) 1.19% to 2.07% for intra-assay and 3.76% to 7.15% for inter-assay precision levels. The recovery rates of IP3G in the leaves of Aquilaria spp. were in the range of 96.0% to 99.0% with CV 4.50% to 5.32%. A correlation between ELISA and high-performance liquid chromatography methods was obtained when analysis of IP3G in the plant samples (R2 = 0.9321). These results suggest that the developed ELISA method can be applied to determine IP3G content with high specificity, rapidity, and simplicity. The developed immunosorbent assay in this study provides a useful tool for the analysis of IP3G in plant samples and products. Practical Application: Agarwood leaves are consumed as health drink due to their proposed anti-inflammatory, anti-diabetic properties, and as a laxative. An indirect competitive enzyme-linked immunosorbent assay method for determination of iriflophenone 3-C-β-d-glucoside showed potential assay for quality control of agarwood leaves raw material and product.

元の言語英語
ページ(範囲)C1363-C1367
ジャーナルJournal of Food Science
78
発行部数9
DOI
出版物ステータス出版済み - 9 1 2013

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Thymelaeaceae
Aquilaria
Glucosides
polyclonal antibodies
glucosides
Enzyme-Linked Immunosorbent Assay
enzyme-linked immunosorbent assay
Antibodies
assays
cross reaction
leaves
laxatives
antibodies
Laxatives
Immunosorbents
iriflophenone
quality control
raw materials
Quality Control
high performance liquid chromatography

All Science Journal Classification (ASJC) codes

  • Food Science

これを引用

Determination of iriflophenone 3-c-β-d-glucoside from aquilaria spp. by an indirect competitive enzyme-linked immunosorbent assay using a specific polyclonal antibody. / Putalun, Waraporn; Yusakul, Gorawit; Saensom, Paritad; Sritularak, Boonchoo; Tanaka, Hiroyuki.

:: Journal of Food Science, 巻 78, 番号 9, 01.09.2013, p. C1363-C1367.

研究成果: ジャーナルへの寄稿記事

Putalun, Waraporn ; Yusakul, Gorawit ; Saensom, Paritad ; Sritularak, Boonchoo ; Tanaka, Hiroyuki. / Determination of iriflophenone 3-c-β-d-glucoside from aquilaria spp. by an indirect competitive enzyme-linked immunosorbent assay using a specific polyclonal antibody. :: Journal of Food Science. 2013 ; 巻 78, 番号 9. pp. C1363-C1367.
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abstract = "Polyclonal antibody against iriflophenone 3-C-β-d-glucoside (IP3G), a major compound from the leaves of Aquilaria spp., was produced for the development of an enzyme-linked immunosorbent assay (ELISA). The results showed that the antibodies were specific for IP3G. The produced antibody has low cross reactivity with iriflophenone 3,5-C-β-d-diglucopyranoside (13{\%}), genkwanin 5-O-β-primeveroside (3.55{\%}) and no cross reactivity found in other compounds. The range of ELISA assay extends from 100 to 1560 ng/mL with coefficient of variation (CV) 1.19{\%} to 2.07{\%} for intra-assay and 3.76{\%} to 7.15{\%} for inter-assay precision levels. The recovery rates of IP3G in the leaves of Aquilaria spp. were in the range of 96.0{\%} to 99.0{\%} with CV 4.50{\%} to 5.32{\%}. A correlation between ELISA and high-performance liquid chromatography methods was obtained when analysis of IP3G in the plant samples (R2 = 0.9321). These results suggest that the developed ELISA method can be applied to determine IP3G content with high specificity, rapidity, and simplicity. The developed immunosorbent assay in this study provides a useful tool for the analysis of IP3G in plant samples and products. Practical Application: Agarwood leaves are consumed as health drink due to their proposed anti-inflammatory, anti-diabetic properties, and as a laxative. An indirect competitive enzyme-linked immunosorbent assay method for determination of iriflophenone 3-C-β-d-glucoside showed potential assay for quality control of agarwood leaves raw material and product.",
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AB - Polyclonal antibody against iriflophenone 3-C-β-d-glucoside (IP3G), a major compound from the leaves of Aquilaria spp., was produced for the development of an enzyme-linked immunosorbent assay (ELISA). The results showed that the antibodies were specific for IP3G. The produced antibody has low cross reactivity with iriflophenone 3,5-C-β-d-diglucopyranoside (13%), genkwanin 5-O-β-primeveroside (3.55%) and no cross reactivity found in other compounds. The range of ELISA assay extends from 100 to 1560 ng/mL with coefficient of variation (CV) 1.19% to 2.07% for intra-assay and 3.76% to 7.15% for inter-assay precision levels. The recovery rates of IP3G in the leaves of Aquilaria spp. were in the range of 96.0% to 99.0% with CV 4.50% to 5.32%. A correlation between ELISA and high-performance liquid chromatography methods was obtained when analysis of IP3G in the plant samples (R2 = 0.9321). These results suggest that the developed ELISA method can be applied to determine IP3G content with high specificity, rapidity, and simplicity. The developed immunosorbent assay in this study provides a useful tool for the analysis of IP3G in plant samples and products. Practical Application: Agarwood leaves are consumed as health drink due to their proposed anti-inflammatory, anti-diabetic properties, and as a laxative. An indirect competitive enzyme-linked immunosorbent assay method for determination of iriflophenone 3-C-β-d-glucoside showed potential assay for quality control of agarwood leaves raw material and product.

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